Please use this identifier to cite or link to this item: https://doi.org/10.1159/000070380
Title: Sleep deprivation inhibits expression of NADPH-d and NOS while activating microglia and astroglia in the rat hippocampus
Authors: Hsu, J.-C.
Lee, Y.-S.
Chang, C.-N.
Chuang, H.-L.
Ling, E.-A. 
Lan, C.-T.
Keywords: CNS
Immunohistochemistry
Memory
Nitric oxide
Rat
Western blot
Issue Date: 2003
Source: Hsu, J.-C., Lee, Y.-S., Chang, C.-N., Chuang, H.-L., Ling, E.-A., Lan, C.-T. (2003). Sleep deprivation inhibits expression of NADPH-d and NOS while activating microglia and astroglia in the rat hippocampus. Cells Tissues Organs 173 (4) : 242-254. ScholarBank@NUS Repository. https://doi.org/10.1159/000070380
Abstract: This study investigated the expression of nitric oxide (NO)-synthesizing enzymes and the glial reaction in the rat hippocampal formation following sleep deprivation for 5 days. Nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) reactivity was markedly reduced in the hippocampal CA1, CA2 and CA3 sectors as well as in the dentate gyrus, suggesting a suppression of NO production in these areas. Microglial cells were hypertrophic and showed an up-regulation of complement type 3 receptors as determined by antibody OX-42. However, expression of major histocompatibility complex class I and II antigens, and antigen of monocyte/macrophage lineage marked by OX-18, OX-6 and ED1, respectively, was undetected. Astrocytes also displayed hypertrophied processes with enhanced glial fibrillary acidic protein (GFAP) immunoreactivity. Western blots of hippocampal tissues corroborated the above-mentioned morphological findings in that expression of NO-synthase (NOS) was decreased while that of OX-42 and GFAP was increased in the sleep-deprived rats. Since NO is thought to be involved in memory consolidation processes in the hippocampus during sleep, the inhibition of NADPH-d and NOS reactivities may account for the memory decline after long-term sleep deprivation. The concomitant reactions in microglia and astrocytes suggest the involvement of these cells in the deleterious effect of prolonged sleep deprivation. Copyright © 2003 S. Karger AG, Basel.
Source Title: Cells Tissues Organs
URI: http://scholarbank.nus.edu.sg/handle/10635/107979
ISSN: 14226405
DOI: 10.1159/000070380
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