Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/107765
Title: Cloning of the aldehyde reductase gene from a red yeast, Sporobolomyces salmonicolor, and characterization of the gene and its product
Authors: Kita, K.
Matsuzaki, K.
Hashimoto, T.
Yanase, H.
Kato, N.
Chung, M.C.-M. 
Kataoka, M.
Shimizu, S.
Issue Date: Jul-1996
Source: Kita, K.,Matsuzaki, K.,Hashimoto, T.,Yanase, H.,Kato, N.,Chung, M.C.-M.,Kataoka, M.,Shimizu, S. (1996-07). Cloning of the aldehyde reductase gene from a red yeast, Sporobolomyces salmonicolor, and characterization of the gene and its product. Applied and Environmental Microbiology 62 (7) : 2303-2310. ScholarBank@NUS Repository.
Abstract: An NADPH-dependent aldehyde reductase (ALR) isolated from a red yeast, Sporobolomyces salmonicolor, catalyzes the reduction of a variety of carbonyl compounds. To investigate its primary structure, we cloned and sequenced the cDNA coding for ALR. The aldehyde reductase gene (ALR) comprises 969 bp and encodes a polypeptide of 35,232 Da. The deduced amino acid sequence showed a high degree of similarity to other members of the aldo-keto reductase superfamily. Analysis of the genomic DNA sequence indicated that the ALR gene was interrupted by six introns (two in the 5' noncoding region and four in the coding region). Southern hybridization analysis of the genomic DNA from S. salmonicolor indicated that there was one copy of the gene. The ALR gene was expressed in Escherichia coli under the control of the tac promoter. The enzyme expressed in E. coli was purified to homogeneity and showed the same catalytic properties as did the enzyme from S. salmonicolor.
Source Title: Applied and Environmental Microbiology
URI: http://scholarbank.nus.edu.sg/handle/10635/107765
ISSN: 00992240
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

25
checked on Feb 17, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.