Transport of thalidomide by the human intestinal Caco-2 monolayers

Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/106468

Title:	Transport of thalidomide by the human intestinal Caco-2 monolayers
Authors:	Zhou, S. Li, Y. Kestell, P. Schafer, P. Chan, E. Paxton, J.W.
Keywords:	Caco-2 Intestinal Permeability Thalidomide
Issue Date:	Jan-2005
Citation:	Zhou, S.,Li, Y.,Kestell, P.,Schafer, P.,Chan, E.,Paxton, J.W. (2005-01). Transport of thalidomide by the human intestinal Caco-2 monolayers. European Journal of Drug Metabolism and Pharmacokinetics 30 (1-2) : 49-61. ScholarBank@NUS Repository.
Abstract:	Studies in patients have indicated that the oral absorption of thalidomide is considerably variable at high doses (>200 mg/day). The aim of this study was to investigate the transport of racemic thalidomide using human colon cancer cell line (Caco-2) monolayers, which have been widely used to investigate drug permeability. A typical 21-day protocol was used to prepare Caco-2 monolayers. Thalidomide was determined by a validated high performance liquid chromatography method with ultraviolet detection. The integrity of Caco-2 monolayer was confirmed when the transepithelial electrical resistance (TEER) exceeded 300 Ω · cm2, and the leakage of 14C-manitol was
Source Title:	European Journal of Drug Metabolism and Pharmacokinetics
URI:	http://scholarbank.nus.edu.sg/handle/10635/106468
ISSN:	03787966
Appears in Collections:	Staff Publications

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