Please use this identifier to cite or link to this item: https://doi.org/10.1080/10826076.2011.572215
Title: Quantification of trans-resveratrol in rat plasma by a simple and sensitive high performance liquid chromatography method and its application in pre-clinical study
Authors: Das, S.
Ng, K.-Y. 
Keywords: HPLC
liqid-liquid extraction
plasma
trans-resveratrol
validation
Issue Date: Jan-2011
Citation: Das, S., Ng, K.-Y. (2011-01). Quantification of trans-resveratrol in rat plasma by a simple and sensitive high performance liquid chromatography method and its application in pre-clinical study. Journal of Liquid Chromatography and Related Technologies 34 (14) : 1399-1414. ScholarBank@NUS Repository. https://doi.org/10.1080/10826076.2011.572215
Abstract: The intention of this study was to develop and validate a simple, sensitive, and accurate high performance liquid chromatography (HPLC) method for quantification of trans-resveratrol in rat plasma. Trans-resveratrol was recovered from plasma samples by liquid-liquid extraction. A reverse phase HPLC column was used and the assay was performed at 35°C through isocratic delivery (1mL·min -1) of acetonitrile and phosphate buffer solution, pH 7.0 (30:70v/v). UV absorbance was monitored at 320nm. Plasma samples were stable (>99%) at -80°C for 3 months. The recovery of trans-resveratrol from plasma was 95%. The HPLC method was validated. The retention times of trans-resveratrol and carbamazepine were 5.5 and 10.4min, respectively. The calibration curve was linear (R 2=0.9999) within the range of 5-1,000ng·mL -1. The lower limits of detection and quantification of trans-resveratrol were 2.5 and 5ng·mL -1, respectively. The intra-day and inter-day variations were
Source Title: Journal of Liquid Chromatography and Related Technologies
URI: http://scholarbank.nus.edu.sg/handle/10635/106280
ISSN: 10826076
DOI: 10.1080/10826076.2011.572215
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