Please use this identifier to cite or link to this item:
|Title:||Quantification of trans-resveratrol in rat plasma by a simple and sensitive high performance liquid chromatography method and its application in pre-clinical study|
|Citation:||Das, S., Ng, K.-Y. (2011-01). Quantification of trans-resveratrol in rat plasma by a simple and sensitive high performance liquid chromatography method and its application in pre-clinical study. Journal of Liquid Chromatography and Related Technologies 34 (14) : 1399-1414. ScholarBank@NUS Repository. https://doi.org/10.1080/10826076.2011.572215|
|Abstract:||The intention of this study was to develop and validate a simple, sensitive, and accurate high performance liquid chromatography (HPLC) method for quantification of trans-resveratrol in rat plasma. Trans-resveratrol was recovered from plasma samples by liquid-liquid extraction. A reverse phase HPLC column was used and the assay was performed at 35°C through isocratic delivery (1mL·min -1) of acetonitrile and phosphate buffer solution, pH 7.0 (30:70v/v). UV absorbance was monitored at 320nm. Plasma samples were stable (>99%) at -80°C for 3 months. The recovery of trans-resveratrol from plasma was 95%. The HPLC method was validated. The retention times of trans-resveratrol and carbamazepine were 5.5 and 10.4min, respectively. The calibration curve was linear (R 2=0.9999) within the range of 5-1,000ng·mL -1. The lower limits of detection and quantification of trans-resveratrol were 2.5 and 5ng·mL -1, respectively. The intra-day and inter-day variations were|
|Source Title:||Journal of Liquid Chromatography and Related Technologies|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 23, 2018
WEB OF SCIENCETM
checked on May 8, 2018
checked on May 18, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.