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Title: Prolonged retention of cross-linked trypsin in calcium alginate microspheres
Authors: Chui, W.K. 
Wan, L.S.C. 
Keywords: Alginate
Issue Date: Jan-1997
Citation: Chui, W.K.,Wan, L.S.C. (1997-01). Prolonged retention of cross-linked trypsin in calcium alginate microspheres. Journal of Microencapsulation 14 (1) : 51-61. ScholarBank@NUS Repository.
Abstract: Trypsin microencapsulated in a calcium alginate matrix was lost quickly through diffusion when the microspheres were placed in an aqueous medium. This problem was overcome by first reacting trypsin with glutaraldehyde to form cross-linkages and then incorporating the enzyme in the alginate microspheres. The performance of the cross-linked trypsin remained optimal at pH 8 while it was found to be more heat-stable and remained highly active even at 80°C. Esters and amides of L-arginine were preferentially hydrolysed by the enzyme indicating that cross-linking did not adversely affect the conformation of the active site. There was a suppression in enzymatic activity when the microspheres were placed in reaction media with an increasing concentration of organic solvent such as ethanol, acetonitrile or isopropanol. However, when returned to a totally aqueous environment, the enzyme resumed its initial tryptic capability. Such a microencapsulated form of cross-linked enzyme may find application in enzyme replacement therapy, optical resolution of racemic compounds as well as organic synthesis in an aqueous-organic environment.
Source Title: Journal of Microencapsulation
ISSN: 02652048
Appears in Collections:Staff Publications

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