Please use this identifier to cite or link to this item:
|Title:||Metabolic profiling of 3-nitropropionic acid early-stage huntingtons disease rat model using gas chromatography time-of-flight mass spectrometry|
|Citation:||Chang, K.L., New, L.S., Mal, M., Goh, C.W., Aw, C.C., Browne, E.R., Chan, E.C.Y. (2011-04-01). Metabolic profiling of 3-nitropropionic acid early-stage huntingtons disease rat model using gas chromatography time-of-flight mass spectrometry. Journal of Proteome Research 10 (4) : 2079-2087. ScholarBank@NUS Repository. https://doi.org/10.1021/pr2000336|
|Abstract:||3-Nitropropionic acid (3-NP), a potent irreversible inhibitor of mitochondrial complex II enzyme, leads to mitochondrial dysfunction and oxidative stress in Huntingtons disease (HD) rat model. In this study, biochemical assays were used to demonstrate the presence of oxidative stress and mitochondrial dysfunction in 3-NP early stage HD rat models. Gas chromatography time-of-flight mass spectrometry (GC/TOFMS) was applied to analyze metabolites in brain and plasma of 3-NP-treated and vehicle-dosed rats. The orthogonal partial least-squares discriminant analysis (OPLS-DA) model generated using brain metabolic profiles robustly differentiated the 3-NP early stage HD rat model from the control. Metabonomic characterization of the 3-NP HD rat model facilitated the detection of biomarkers that define the physiopathological phenotype of early stage HD and elucidated the treatment effect of galantamine. Brain marker metabolites that were identified based on the OPLS-DA model were associated with altered glutathione metabolism, oxidative stress, and impaired energy metabolism. The treatment effect of galantamine in early stage HD could not be concluded mechanistically using the brain metabotype. Our study confirmed that GC/TOFMS is a strategic and complementary platform for the metabonomic characterization of 3-NP induced neurotoxicity in the early stage HD rat model. © 2011 American Chemical Society.|
|Source Title:||Journal of Proteome Research|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jun 14, 2018
WEB OF SCIENCETM
checked on May 15, 2018
checked on May 11, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.