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|Title:||High-throughput determination of carbocysteine in human plasma by liquid chromatography/tandem mass spectrometry: Application to a bioequivalence study of two formulations in healthy volunteers|
|Citation:||Bi, H.-C., Zhao, L.-Z., Zhong, G.-P., Zhou, S., Li, B., Deng, Y., Chen, X., Huang, M. (2006). High-throughput determination of carbocysteine in human plasma by liquid chromatography/tandem mass spectrometry: Application to a bioequivalence study of two formulations in healthy volunteers. Rapid Communications in Mass Spectrometry 20 (7) : 1153-1160. ScholarBank@NUS Repository. https://doi.org/10.1002/rcm.2422|
|Abstract:||A rapid and sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method to determine carbocysteine in human plasma was developed and fully validated. After methanol-induced protein precipitation of the plasma samples, carbocysteine was subjected to LC/MS/MS analysis using electrospray ionization (ESI). The MS system was operated in the selected ion monitoring (SRM) mode. Chromatographic separation was performed on a Hypurity C 18 column (i.d. 2.1 mm x 50 mm, particle size 5 μm). The method had a chromatographic running time of 2.0 min and linear calibration curves over the concentration ranges of 0.1-20 μg/mL for carbocysteine. The lower limit of quantification (LLOQ) of the method was 0.1 μg/mL for carbocysteine. The intra- and inter-day precision was less than 7% for all quality control samples at concentrations of 0.5, 2.0, and 10.0 μg/mL. These results indicate that the method was efficient with a simple preparation procedure and a very short running time (2.0 min) for carbocysteine compared with methods reported in the literature and had high selectivity, acceptable accuracy, precision and sensitivity. The validated LC/ MS/MS method has been successfully used to a bioequivalence study of two tablet formulations of carbocysteine in healthy volunteers. Copyright © 2006 John Wiley & Sons, Ltd.|
|Source Title:||Rapid Communications in Mass Spectrometry|
|Appears in Collections:||Staff Publications|
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