Please use this identifier to cite or link to this item: https://doi.org/10.1002/1097-0231(20001115)14:213.0.CO;2-T
Title: High-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometric method for the analysis of catecholamines and metanephrines in human urine
Authors: Chan, E.C.Y. 
Ho, P.C. 
Issue Date: 2000
Source: Chan, E.C.Y.,Ho, P.C. (2000). High-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometric method for the analysis of catecholamines and metanephrines in human urine. Rapid Communications in Mass Spectrometry 14 (21) : 1959-1964. ScholarBank@NUS Repository. https://doi.org/10.1002/1097-0231(20001115)14:213.0.CO;2-T
Abstract: An assay of norepinephrine (NE), epinephrine (E), dopamine (DA), normetanephrine (NE) and metanephrine (MN) based on high-performance liquid chromatography (HPLC) in combination with atmospheric pressure chemical ionization mass spectrometry (APcI-MS) is described. The catecholamines and metanephrines were extracted from urine and aqueous samples using Bio-Rex 70 cation-exchange resin and subjected to analysis by HPLC/APcI-MS. The separation was performed on a C18 column in 50 mM ammonium formate buffer, pH 3.0, using a flow rate of 0.8 mL/min. Acetonitrile was added post-column at a flow rate of 0.2 mL/min via a post-column addition tee. The total analysis time was 6.5 min. The quantitative analysis was performed using 3,4-dihydroxybenzylamine (DHBA) as the internal standard (I.S.). Selected ion monitoring detection was applied: m/z 170 (for NE), 184. (for E and NM), 154 (for DA), 198 (for MN) and 140 (for DHBA, I.S.). The limits of quantitation were 5 ng/mL for NE, E and DA and 2.5 ng/mL for NM and MN. The recovery ranged from 75 to 83 % for each analyte. The method was found to be simple and highly selective for the determination of catecholamines and metanephrines in the urine of patients suspected of pheochromocytoma. Copyright (C) 2000 John Wiley and Sons, Ltd.
Source Title: Rapid Communications in Mass Spectrometry
URI: http://scholarbank.nus.edu.sg/handle/10635/105998
ISSN: 09514198
DOI: 10.1002/1097-0231(20001115)14:213.0.CO;2-T
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

21
checked on Feb 24, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.