Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2007.11.021
Title: Efficient intracellular delivery of functional proteins using cationic polymer core/shell nanoparticles
Authors: Lee, A.L.Z.
Wang, Y.
Ye, W.-H.
Yoon, H.S.
Chan, S.Y. 
Yang, Y.-Y.
Keywords: Cationic core/shell nanoparticles
Intracellular delivery
Lectin
Proteins
Issue Date: Mar-2008
Citation: Lee, A.L.Z., Wang, Y., Ye, W.-H., Yoon, H.S., Chan, S.Y., Yang, Y.-Y. (2008-03). Efficient intracellular delivery of functional proteins using cationic polymer core/shell nanoparticles. Biomaterials 29 (9) : 1224-1232. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2007.11.021
Abstract: Cationic core/shell nanoparticles self-assembled from biodegradable, cationic and amphiphilic copolymer poly{N-methyldietheneamine sebacate)-co-[(cholesteryl oxocarbonylamido ethyl) methyl bis(ethylene) ammonium bromide] sebacate}, P(MDS-co-CES), were fabricated and employed to deliver lectin A-chain, an anticancer glycoprotein. Lectin A-chain was efficiently bound onto the surfaces of the nanoparticles at high mass ratios of nanoparticles to lectin A-chain. The nanoparticle/lectin A-chain complexes had an average size of approximately 150 nm with zeta potential of about +30 mV at the mass ratio of 50 or above while the BioPorter/lectin A-chain complexes had a larger particle size and relatively lower zeta potential (150 nm vs. 455 nm; +30 mV vs. +20 mV). Therefore, the cellular uptake of nanoparticle/lectin A-chain complexes was much greater than that of BioPorter/lectin A-chain complexes. The results obtained from cytotoxicity tests show that lectin A-chain delivered by the nanoparticles was significantly more toxic against MDA-MB-231, HeLa, HepG2 and 4T1 cell lines when compared to BioPorter, and IC50 of lectin A-chain delivered by the nanoparticles was 0.2, 0.5, 10 and 50 mg/l, respectively, while that of lectin A-chain delivered by BioPorter was higher than 100 mg/l in all cell lines tested. These nano-sized particles may provide an efficient approach for intracellular delivery of biologically active proteins. © 2007 Elsevier Ltd. All rights reserved.
Source Title: Biomaterials
URI: http://scholarbank.nus.edu.sg/handle/10635/105913
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2007.11.021
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