Please use this identifier to cite or link to this item:
https://doi.org/10.1016/j.biomaterials.2007.11.021
Title: | Efficient intracellular delivery of functional proteins using cationic polymer core/shell nanoparticles | Authors: | Lee, A.L.Z. Wang, Y. Ye, W.-H. Yoon, H.S. Chan, S.Y. Yang, Y.-Y. |
Keywords: | Cationic core/shell nanoparticles Intracellular delivery Lectin Proteins |
Issue Date: | Mar-2008 | Citation: | Lee, A.L.Z., Wang, Y., Ye, W.-H., Yoon, H.S., Chan, S.Y., Yang, Y.-Y. (2008-03). Efficient intracellular delivery of functional proteins using cationic polymer core/shell nanoparticles. Biomaterials 29 (9) : 1224-1232. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2007.11.021 | Abstract: | Cationic core/shell nanoparticles self-assembled from biodegradable, cationic and amphiphilic copolymer poly{N-methyldietheneamine sebacate)-co-[(cholesteryl oxocarbonylamido ethyl) methyl bis(ethylene) ammonium bromide] sebacate}, P(MDS-co-CES), were fabricated and employed to deliver lectin A-chain, an anticancer glycoprotein. Lectin A-chain was efficiently bound onto the surfaces of the nanoparticles at high mass ratios of nanoparticles to lectin A-chain. The nanoparticle/lectin A-chain complexes had an average size of approximately 150 nm with zeta potential of about +30 mV at the mass ratio of 50 or above while the BioPorter/lectin A-chain complexes had a larger particle size and relatively lower zeta potential (150 nm vs. 455 nm; +30 mV vs. +20 mV). Therefore, the cellular uptake of nanoparticle/lectin A-chain complexes was much greater than that of BioPorter/lectin A-chain complexes. The results obtained from cytotoxicity tests show that lectin A-chain delivered by the nanoparticles was significantly more toxic against MDA-MB-231, HeLa, HepG2 and 4T1 cell lines when compared to BioPorter, and IC50 of lectin A-chain delivered by the nanoparticles was 0.2, 0.5, 10 and 50 mg/l, respectively, while that of lectin A-chain delivered by BioPorter was higher than 100 mg/l in all cell lines tested. These nano-sized particles may provide an efficient approach for intracellular delivery of biologically active proteins. © 2007 Elsevier Ltd. All rights reserved. | Source Title: | Biomaterials | URI: | http://scholarbank.nus.edu.sg/handle/10635/105913 | ISSN: | 01429612 | DOI: | 10.1016/j.biomaterials.2007.11.021 |
Appears in Collections: | Staff Publications |
Show full item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.