Please use this identifier to cite or link to this item:
|Title:||Dynamic quantitative photothermal monitoring of cell death of individual human red blood cells upon glucose depletion|
|Keywords:||Cell death monitoring|
Linear response modeling and curve-fitting
|Citation:||Vasudevan, S., Chen, G.C.K., Andika, M., Agarwal, S., Chen, P., Olivo, M. (2010-09). Dynamic quantitative photothermal monitoring of cell death of individual human red blood cells upon glucose depletion. Journal of Biomedical Optics 15 (5) : -. ScholarBank@NUS Repository. https://doi.org/10.1117/1.3484260|
|Abstract:||Red blood cells (RBCs) have been found to undergo "programmed cell death," or eryptosis, and understanding this process can provide more information about apoptosis of nucleated cells. Photothermal (PT) response, a label-free photothermal noninvasive technique, is proposed as a tool to monitor the cell death process of living human RBCs upon glucose depletion. Since the physiological status of the dying cells is highly sensitive to photothermal parameters (e.g., thermal diffusivity, absorption, etc.), we applied linear PT response to continuously monitor the death mechanism of RBC when depleted of glucose. The kinetics of the assay where the cell's PT response transforms from linear to nonlinear regime is reported. In addition, quantitative monitoring was performed by extracting the relevant photothermal parameters from the PT response. Twofold increases in thermal diffusivity and size reduction were found in the linear PT response during cell death. Our results reveal that photothermal parameters change earlier than phosphatidylserine externalization (used for fluorescent studies), allowing us to detect the initial stage of eryptosis in a quantitative manner. Hence, the proposed tool, in addition to detection of eryptosis earlier than fluorescence, could also reveal physiological status of the cells through quantitative photothermal parameter extraction. © 2010 Society of Photo-Optical Instrumentation Engineers.|
|Source Title:||Journal of Biomedical Optics|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Aug 17, 2018
WEB OF SCIENCETM
checked on Aug 8, 2018
checked on Jun 8, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.