Please use this identifier to cite or link to this item: https://doi.org/10.1002/rcm.1607
Title: Determination of tributyrin and its metabolite butyrate in Wistar rat plasma samples by gas chromatography/mass spectrometry
Authors: Su, J.
Zhang, N.
Ho, P.C. 
Issue Date: 2004
Source: Su, J., Zhang, N., Ho, P.C. (2004). Determination of tributyrin and its metabolite butyrate in Wistar rat plasma samples by gas chromatography/mass spectrometry. Rapid Communications in Mass Spectrometry 18 (19) : 2217-2222. ScholarBank@NUS Repository. https://doi.org/10.1002/rcm.1607
Abstract: A gas chromatographic (GC) method with mass spectrometric (MS) detection was developed for the determination of tributyrin and its metabolite butyrate in rat plasma. Following precipitation of plasma protein with acetonitrile, the analytes in the samples were separated on a DB-5ms capillary column with helium as carrier gas. Phenylmethylsulfonyl fluoride (PMSF), an inhibitor for serine proteases, papain and acetylcholinesterase, was found to be essential to inhibit the activity of enzyme(s) responsible for the hydrolysis of tributyrin in both rat and human blood samples. The enzyme inhibitor in 5 mM (final concentration) was added immediately into the blood samples after collection to prevent the hydrolysis. The linear concentration ranges for tributyrin and butyrate were 0.1-2.0 and 1-20 μM, respectively. The coefficients of variation for intra-day and inter-day assays for tributyrin were all
Source Title: Rapid Communications in Mass Spectrometry
URI: http://scholarbank.nus.edu.sg/handle/10635/105812
ISSN: 09514198
DOI: 10.1002/rcm.1607
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