Please use this identifier to cite or link to this item: https://doi.org/10.1128/JB.01253-09
Title: The cyclic nucleotide monophosphate domain of Xanthomonas campestris global regulator Clp defines a new class of cyclic di-GMP effectors
Authors: Tao, F.
He, Y.-W.
Wu, D.-H.
Swarup, S. 
Zhang, L.-H. 
Issue Date: Feb-2010
Citation: Tao, F., He, Y.-W., Wu, D.-H., Swarup, S., Zhang, L.-H. (2010-02). The cyclic nucleotide monophosphate domain of Xanthomonas campestris global regulator Clp defines a new class of cyclic di-GMP effectors. Journal of Bacteriology 192 (4) : 1020-1029. ScholarBank@NUS Repository. https://doi.org/10.1128/JB.01253-09
Abstract: The widely conserved second messenger cyclic diguanosine monophosphate (c-di-GMP) plays a key role in quorum-sensing (QS)-dependent production of virulence factors in Xanthomonas campestris pv. campestris. The detection of QS diffusible signal factor (DSF) by the sensor RpfC leads to the activation of response regulator RpfG, which activates virulence gene expression by degrading c-di-GMP. Here, we show that a global regulator in the X. campestris pv. campestris QS regulatory pathway, Clp, is a c-di-GMP effector. c-di-GMP specifically binds to Clp with high affinity and induces allosteric conformational changes that abolish the interaction between Clp and its target gene promoter. Clp is similar to the cyclic AMP (cAMP) binding proteins Crp and Vfr and contains a conserved cyclic nucleotide monophosphate (cNMP) binding domain. Using site-directed mutagenesis, we found that the cNMP binding domain of Clp contains a glutamic acid residue (E99) that is essential for c-di-GMP binding. Substituting the residue with serine (E99S) resulted in decreased sensitivity to changes in the intracellular c-di-GMP level and attenuated bacterial virulence. These data establish the direct role of Clp in the response to fluctuating c-di-GMP levels and depict a novel mechanism by which QS links the second messenger with the X. campestris pv. campestris virulence regulon. Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Source Title: Journal of Bacteriology
URI: http://scholarbank.nus.edu.sg/handle/10635/101889
ISSN: 00219193
DOI: 10.1128/JB.01253-09
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

98
checked on Jun 14, 2018

WEB OF SCIENCETM
Citations

93
checked on May 9, 2018

Page view(s)

21
checked on Mar 11, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.