Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.str.2010.09.014
Title: Structural and functional analysis of phosphothreonine-dependent FHA domain interactions
Authors: Pennell, S.
Westcott, S.
Ortiz-Lombardía, M.
Patel, D.
Li, J.
Nott, T.J.
Mohammed, D.
Buxton, R.S.
Yaffe, M.B.
Verma, C. 
Smerdon, S.J.
Issue Date: 8-Dec-2010
Citation: Pennell, S., Westcott, S., Ortiz-Lombardía, M., Patel, D., Li, J., Nott, T.J., Mohammed, D., Buxton, R.S., Yaffe, M.B., Verma, C., Smerdon, S.J. (2010-12-08). Structural and functional analysis of phosphothreonine-dependent FHA domain interactions. Structure 18 (12) : 1587-1595. ScholarBank@NUS Repository. https://doi.org/10.1016/j.str.2010.09.014
Abstract: FHA domains are well established as phospho-dependent binding modules mediating signal transduction in Ser/Thr kinase signaling networks in both eukaryotic and prokaryotic species. Although they are unique in binding exclusively to phosphothreonine, the basis for this discrimination over phosphoserine has remained elusive. Here, we attempt to dissect overall binding specificity at the molecular level. We first determined the optimal peptide sequence for Rv0020c FHA domain binding by oriented peptide library screening. This served as a basis for systematic mutagenic and binding analyses, allowing us to derive relative thermodynamic contributions of conserved protein and peptide residues to binding and specificity. Structures of phosphopeptide-bound and uncomplexed Rv0020c FHA domain then directed molecular dynamics simulations which show how the extraordinary discrimination in favor of phosphothreonine occurs through formation of additional hydrogen-bonding networks that are ultimately stabilized by van der Waals interactions of the phosphothreonine γ-methyl group with a conserved pocket on the FHA domain surface. © 2010 Elsevier Ltd. All rights reserved.
Source Title: Structure
URI: http://scholarbank.nus.edu.sg/handle/10635/101735
ISSN: 09692126
DOI: 10.1016/j.str.2010.09.014
Appears in Collections:Staff Publications

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