Sequence-Specific Assignments of Methyl Groups in High-Molecular Weight Proteins

Please use this identifier to cite or link to this item: https://doi.org/10.1021/ja039102q

Title:	Sequence-Specific Assignments of Methyl Groups in High-Molecular Weight Proteins
Authors:	Yang, D. Zheng, Y. Liu, D. Wyss, D.F.
Issue Date:	31-Mar-2004
Citation:	Yang, D., Zheng, Y., Liu, D., Wyss, D.F. (2004-03-31). Sequence-Specific Assignments of Methyl Groups in High-Molecular Weight Proteins. Journal of the American Chemical Society 126 (12) : 3710-3711. ScholarBank@NUS Repository. https://doi.org/10.1021/ja039102q
Abstract:	A new 3D multiple-quantum (H)CCmHm-TOCSY experiment is proposed to assign methyl resonances in high-molecular weight proteins, on the basis of spectral patterns and prior backbone assignments. The favorable relaxation properties of the multiple-quantum coherences and the slow decays of in-phase methyl 13C magnetizations optimize performance of the proposed experiment for application to large proteins. The experiment has been demonstrated on an acyl carrier protein synthase (trimer, 42 kDa, overall correlation time of 26 ns) at 25 °C, and 63 out of 67 nonmethionine methyl groups have been assigned. Copyright © 2004 American Chemical Society.
Source Title:	Journal of the American Chemical Society
URI:	http://scholarbank.nus.edu.sg/handle/10635/101651
ISSN:	00027863
DOI:	10.1021/ja039102q
Appears in Collections:	Staff Publications

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