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|Title:||Quantitative and temporal proteome analysis of butyrate-treated colorectal cancer cells|
|Authors:||Tan, H.T. |
|Citation:||Tan, H.T., Tan, S., Lin, Q., Lim, T.K., Hew, C.L., Chung, M.C.M. (2008-06). Quantitative and temporal proteome analysis of butyrate-treated colorectal cancer cells. Molecular and Cellular Proteomics 7 (6) : 1174-1185. ScholarBank@NUS Repository. https://doi.org/10.1074/mcp.M700483-MCP200|
|Abstract:||Colorectal cancer is one of the most common cancers in developed countries, and its incidence is negatively associated with high dietary fiber intake. Butyrate, a short-chain fatty acid fermentation by-product of fiber induces cell maturation with the promotion of growth arrest, differentiation, and/or apoptosis of cancer cells. The stimulation of cell maturation by butyrate in colonic cancer cells follows a temporal progression from the early phase of growth arrest to the activation of apoptotic cascades. Previously we performed two-dimensional DIGE to identify differentially expressed proteins induced by 24-h butyrate treatment of HCT-116 colorectal cancer cells. Herein we used quantitative proteomics approaches using iTRAQ (isobaric tags for relative and absolute quantitation), a stable isotope labeling methodology that enables multiplexing of four samples, for a temporal study of HCT-116 cells treated with butyrate. In addition, cleavable ICAT, which selectively tags cysteine-containing proteins, was also used, and the results complemented those obtained from the iTRAQ strategy. Selected protein targets were validated by real time PCR and Western blotting. A model is proposed to illustrate our findings from this temporal analysis of the butyrate-responsive proteome that uncovered several integrated cellular processes and pathways involved in growth arrest, apoptosis, and metastasis. These signature clusters of butyrate-regulated pathways are potential targets for novel chemopreventive and therapeutic drugs for treatment of colorectal cancer. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.|
|Source Title:||Molecular and Cellular Proteomics|
|Appears in Collections:||Staff Publications|
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