Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.cbpa.2008.06.004
Title: Lipid, ketone body and oxidative metabolism in the African lungfish, Protopterus dolloi following 60 days of fasting and aestivation
Authors: Frick, N.T.
Bystriansky, J.S.
Ip, Y.K. 
Chew, S.F.
Ballantyne, J.S.
Keywords: Aerobic metabolism
Aestivation
Cytochrome c oxidase
Ketone body metabolism
Lipid metabolism
Metabolic depression
Protopterus
Issue Date: Sep-2008
Citation: Frick, N.T., Bystriansky, J.S., Ip, Y.K., Chew, S.F., Ballantyne, J.S. (2008-09). Lipid, ketone body and oxidative metabolism in the African lungfish, Protopterus dolloi following 60 days of fasting and aestivation. Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology 151 (1) : 93-101. ScholarBank@NUS Repository. https://doi.org/10.1016/j.cbpa.2008.06.004
Abstract: The potential importance of lipids and ketone bodies as fuels in the African lungfish, Protopterus dolloi, and the role of oxidative metabolism, were examined under control, fasted and aestivated conditions. In aestivating but not fasting lungfish, the activities of citrate synthase (CS) and cytochrome c oxidase (CCO) (enzymes of oxidative metabolism) showed tissue-specific changes. Significant reductions in CS activity occurred in the kidney, heart, gill and muscle, and in CCO in the liver and kidney tissues. Aestivation, but not fasting, also had a tissue-specific effect on mitochondrial state 3 respiration rates (using succinate as a substrate), with a > 50% reduction in the liver, yet no change within muscle mitochondria. There is no indication that enzymes involved in lipid catabolism are up-regulated during periods of fasting or aestivation; however, both 3-hydroxyacyl CoA dehydrogenase (HOAD) and carnitine palmitoyl CoA transferase (CPT) activities were sustained in the liver despite the ∼ 42% reduction in CCO activity, potentially indicating lipid metabolism is of importance during aestivation. Lungfish are able to utilize both the d- and l-stereoisomers of the ketone body β-hydroxybutyrate (β-HB); however, β-HB does not appear to be an important fuel source during aestivation or fasting as no changes were observed in β-HB tissue levels. This study demonstrates that an important aspect of metabolic depression during aestivation in lungfish is the tissue-specific down regulation of enzymes of aerobic metabolism while maintaining the activities of enzymes in pathways that supply substrates for aerobic metabolism. © 2008 Elsevier Inc. All rights reserved.
Source Title: Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology
URI: http://scholarbank.nus.edu.sg/handle/10635/101021
ISSN: 10956433
DOI: 10.1016/j.cbpa.2008.06.004
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