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|Title:||Large scale gene expression profiling of metabolic shift of mammalian cells in culture|
De Leon Gatti, M.
Yin Lau, A.L.
Eng Lim, J.W.
Ming Chung, M.C.
Two-dimensional gel electrophoresis
|Source:||Korke, R., De Leon Gatti, M., Yin Lau, A.L., Eng Lim, J.W., Seow, T.K., Ming Chung, M.C., Hu, W.-S. (2004-01-08). Large scale gene expression profiling of metabolic shift of mammalian cells in culture. Journal of Biotechnology 107 (1) : 1-17. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jbiotec.2003.09.007|
|Abstract:||The metabolic state of hybridoma cells in continuous culture varies with the cultivation condition from which the culture is initiated. At a metabolically shifted state, cells have markedly reduced glucose and other nutrient consumption and lactate production as compared to cells in batch culture or in continuous culture without a metabolic shift. Taking a combined genomics and proteomics approach, we investigated the molecular mechanism of metabolic shift. Cells from continuous cultures at two different steady states with a glucose consumption to lactate production molar ratio (ΔL/ΔG) of 0.08 and 1.4 were studied. Affymetrix GeneChips as well as cDNA microarrays were employed to identify differentially expressed mRNA transcripts, and the differentially expressed proteins were identified using the 2D gel electrophoresis-mass spectrometry approach. The decrease in glucose metabolism upon metabolic shift is accompanied by a decrease in gene expression of a number of genes involved in its metabolism. However, the number of genes differentially expressed and the extent of differential expression upon metabolic shift are relatively moderate. The change in the expression of metabolic genes at the transcriptional level was confirmed by real time PCR. The results suggest that metabolic shift is a combined effect of both biochemical events at reaction level and gene expression at transcription and translation level. This approach of integrating transcriptional profiling, proteomic techniques and biochemical analysis provides a more global view of the metabolism of mammalian cells in culture. © 2003 Elsevier B.V. All rights reserved.|
|Source Title:||Journal of Biotechnology|
|Appears in Collections:||Staff Publications|
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