Please use this identifier to cite or link to this item: https://doi.org/10.1159/000082357
Title: Isolation and characterization of the follicle-stimulating hormone β subunit gene and 5′ flanking region of the Chinook salmon
Authors: Chong, K.L.
Wang, S. 
Melamed, P. 
Keywords: Fish
Gene sequencing
Gonadotropins
Molecular neuroendocrinology
Transcription factors
Issue Date: 2004
Citation: Chong, K.L., Wang, S., Melamed, P. (2004). Isolation and characterization of the follicle-stimulating hormone β subunit gene and 5′ flanking region of the Chinook salmon. Neuroendocrinology 80 (3) : 158-170. ScholarBank@NUS Repository. https://doi.org/10.1159/000082357
Abstract: In this study we have isolated the follicle-stimulating hormone β subunit gene from the Chinook salmon (csFSHβ). This gene encodes for a protein that is highly similar to those isolated from other salmonids and shares all of the structural constraints seen in mammalian gonadotropins, including twelve conserved cysteines and a putative N-linked glycosylation site. The organization of the gene follows the conserved pattern regarding the numbers and positions of the introns, although the csFSHβ gene contains a particularly large 6.2-kb first intron due to the inclusion of several transposon-like elements. Isolation of 1.2 kb of the 5′ flanking region of the csFSHβ gene and subsequent analysis in silico have revealed a number of putative elements which appear highly conserved in teleost FSHβ gene promoters and are thus likely involved in basal and hormone-induced transcriptional regulation. The functionality of this 1.2-kb fragment in driving expression of a reporter gene and its response to GnRH was shown in gonadotropes, while the overexpression of AP-1 factors, Sf-1, estrogen receptor or Smad1 revealed that the promoter is responsive to these transcription factors. Our current study has opened the way for future analysis to verify the role of these factors in mediating hormonally induced transcription of this gene. Copyright © 2004 S. Karger AG, Basel.
Source Title: Neuroendocrinology
URI: http://scholarbank.nus.edu.sg/handle/10635/100981
ISSN: 00283835
DOI: 10.1159/000082357
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

11
checked on Jul 17, 2018

WEB OF SCIENCETM
Citations

10
checked on Jul 17, 2018

Page view(s)

45
checked on Jun 1, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.