Please use this identifier to cite or link to this item:
|Title:||Interordinal chimera formation between medaka and zebrafish for analyzing stem cell differentiation|
|Source:||Hong, N., Chen, S., Ge, R., Song, J., Yi, M., Hong, Y. (2012-08-10). Interordinal chimera formation between medaka and zebrafish for analyzing stem cell differentiation. Stem Cells and Development 21 (12) : 2333-2341. ScholarBank@NUS Repository. https://doi.org/10.1089/scd.2011.0630|
|Abstract:||Chimera formation is a standard test for pluripotency of stem cells in vivo. Interspecific chimera formation between distantly related organisms offers also an attractive approach for propagating endangered species. Parameters influencing interspecies chimera formation have remained poorly elucidated. Here, we report interordinal chimera formation between medaka and zebrafish, which separated ∼320 million years ago and exhibit a more than 2-fold difference in developmental speed. We show that, on transplantation into zebrafish blastulae, both noncultivated blastomeres and long-term cultivated embryonic stem (ES) cells of medaka adopted the zebrafish developmental program and differentiated into physiologically functional cell types including pigment cells, blood cells, and cardiomyocytes. We also show that medaka ES cells express differentiation gene markers during chimeric embryogenesis. Therefore, the evolutionary distance and different embryogenesis speeds do not produce donor-host incompatibility to compromise chimera formation between medaka and zebrafish, and molecular markers are valuable for analyzing lineage commitment and cell differentiation in interspecific chimeric embryos. © 2012, Mary Ann Liebert, Inc.|
|Source Title:||Stem Cells and Development|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jan 17, 2018
WEB OF SCIENCETM
checked on Dec 11, 2017
checked on Jan 14, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.