Please use this identifier to cite or link to this item: https://doi.org/10.1007/s00299-008-0551-8
Title: Induction of in vitro flowering in Dendrobium Madame Thong-In (Orchidaceae) seedlings is associated with increase in endogenous N6- (Δ2-isopentenyl)-adenine (iP) and N6- (Δ2-isopentenyl)-adenosine (iPA) levels
Authors: Sim, G.E.
Goh, C.J. 
Loh, C.S. 
Keywords: Dendrobium Madame Thong-In
Endogenous cytokinins
HPLC/Radioimmunoassay
In vitro flowering
Tropical orchid
Issue Date: Aug-2008
Citation: Sim, G.E., Goh, C.J., Loh, C.S. (2008-08). Induction of in vitro flowering in Dendrobium Madame Thong-In (Orchidaceae) seedlings is associated with increase in endogenous N6- (Δ2-isopentenyl)-adenine (iP) and N6- (Δ2-isopentenyl)-adenosine (iPA) levels. Plant Cell Reports 27 (8) : 1281-1289. ScholarBank@NUS Repository. https://doi.org/10.1007/s00299-008-0551-8
Abstract: We analysed the endogenous cytokinin levels of Dendrobium Madame Thong-In seedlings grown in vitro during vegetative and flowering-inductive periods. HPLC was used to fractionate the extracts and radioimmunoassay (RIA) was used for assay of zeatin (Z), dihydrozeatin (DZ), N6-(Δ2- isopentenyl)-adenine (iP) and their derivatives. Coconut water used in experiments was found to contain high level (>136 pmol ml-1) of zeatin riboside (ZR). Protocorms and seedlings cultured in medium with coconut water were found to contain 0.5-3.9 pmol g-1 FW of the cytokinins analysed. Seedlings (1.0-1.5 cm) cultured in flowering-inductive liquid medium containing 6-benzyladenine (BA, 4.4 μM) and coconut water (CW, 15%) contained up to 200 and 133 pmol g-1 FW of iP and iPA, respectively. These levels were significantly higher than all other cytokinins analysed in seedlings of the same stage and were about 80- to 150-folds higher than seedlings cultured in non-inductive medium. During the transitional (vegetative to reproductive) stage, the endogenous levels of iP (178 pmol g-1 FW) and iPA (63 pmol g-1 FW) were also significantly higher than cytokinins in the zeatine (Z) and dihydrozeatin (DZ) families in the same seedlings. Seedlings that grew on inductive medium but remained vegetative contained lower levels of iPA. The importance of the profiles of iP and its derivatives in induction of in vitro flowering of D. Madame Thong-In is discussed. © 2008 Springer-Verlag.
Source Title: Plant Cell Reports
URI: http://scholarbank.nus.edu.sg/handle/10635/100920
ISSN: 07217714
DOI: 10.1007/s00299-008-0551-8
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

14
checked on Sep 19, 2018

WEB OF SCIENCETM
Citations

9
checked on Sep 5, 2018

Page view(s)

34
checked on Sep 14, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.