Please use this identifier to cite or link to this item:
Title: High-efficiency transient transduction of human embryonic stem cell-derived neurons with baculoviral vectors
Authors: Zeng, J.
Du, J.
Lin, J.
Bak, X.Y.
Wu, C.
Wang, S. 
Issue Date: 2009
Source: Zeng, J., Du, J., Lin, J., Bak, X.Y., Wu, C., Wang, S. (2009). High-efficiency transient transduction of human embryonic stem cell-derived neurons with baculoviral vectors. Molecular Therapy 17 (9) : 1585-1593. ScholarBank@NUS Repository.
Abstract: Transient genetic manipulation of human neurons without chromosomal integration of the transgene would be valuable but has been challenging due to the quiescent nature of these postmitotic cells. In this study, we developed a set of baculoviral vectors for transient transduction in nondividing neurons derived from human embryonic stem cells (hESCs). Using a baculoviral vector equipped with the woodchuck hepatitis virus posttranscriptional regulatory element (WPRE), we observed a quick onset of transgene expression as early as day 1 after baculoviral transduction and a high efficiency of up to 80%. Strong transgene expression in the cultured human neurons was observed for more than 1 month and the signal was easily detectable even after 3 months. Using two baculoviral vectors carrying different transgenes, we found that co-transduction at a single neuron level was possible. After transplantation into the brain of nude mice, the baculovirus-transduced human neurons were integrated into the mouse brain and maintained transgene expression for at least 4 weeks, portending the usefulness of this technique in assisting neural transplantation. Therefore, by mediating efficient transient gene expression, baculoviral vectors can provide useful tools for both basic gene function studies in human neurons and therapeutic applications of these cells.
Source Title: Molecular Therapy
ISSN: 15250016
DOI: 10.1038/mt.2009.124
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Mar 6, 2018


checked on Mar 6, 2018

Page view(s)

checked on Feb 25, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.