Please use this identifier to cite or link to this item: https://doi.org/10.1007/s10126-002-0007-z
Title: Genetic diversity within and among feral populations and domesticated strains of the guppy (Poecilia reticulata) in Singapore
Authors: Khoo, G. 
Lim, K.F.
Gan, D.K.Y.
Chen, F.
Chan, W.-K. 
Lim, T.M. 
Phang, V.P.E. 
Keywords: Cluster analysis
Genetic differentiation
Guppy
Poecilia reticulata
RAPD-PCR
Issue Date: Jul-2002
Source: Khoo, G., Lim, K.F., Gan, D.K.Y., Chen, F., Chan, W.-K., Lim, T.M., Phang, V.P.E. (2002-07). Genetic diversity within and among feral populations and domesticated strains of the guppy (Poecilia reticulata) in Singapore. Marine Biotechnology 4 (4) : 367-378. ScholarBank@NUS Repository. https://doi.org/10.1007/s10126-002-0007-z
Abstract: Genetic variability within and among feral populations and cultured strains of the guppy (Poecilia reticulata) was investigated by random amplification of polymorphic DNA (RAPD) fingerprinting. Feral guppies were collected from 6 isolated populations (BT, Bukit Timah; NS, Nee Soon; TS, Tuas; MF, Mount Faber; KR, Kranji; LI, laboratory-inbred feral line), while the Tuxedo and Green Variegated strains were sampled from 2 guppy farms in Singapore. Pairwise genetic distances analyzed by unweighted pair-group method with arithmetic means revealed distinct clustering of guppy individuals into their respective populations and strains. Percentage polymorphic loci ranged from 54.96% (TS) to 68.70% (KR), while average heterozygosity ranged from 0.220 (GV) to 0.271 (KR). In contrast, TS guppies had the highest (0.850) intrapopulation genetic similarity (S), whereas KR had the lowest (0.781). Among populations and strains, S ranged from 0.703 (between GV and LI) to 0.809 (between NS and MB). The GV strain S was closer to TX (0.784) than to the feral guppies. Bootstrapped genetic distance trees depicted 3 major nodes comprising BT-TS, NS-MF, and TX-GV. Principal coordinate analysis also differentiated the 6 feral populations from the 2 cultured strains.
Source Title: Marine Biotechnology
URI: http://scholarbank.nus.edu.sg/handle/10635/100750
ISSN: 14362228
DOI: 10.1007/s10126-002-0007-z
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