Please use this identifier to cite or link to this item:
Title: Early in vitro flowering and seed production in culture in Dendrobium Chao Praya Smile (Orchidaceae)
Authors: Hee, K.H.
Loh, C.S. 
Yeoh, H.H. 
Keywords: Dendrobium Chao Praya Smile
In vitro flowering
Pollen germination
Seed production in culture
Sporad analysis
Issue Date: Dec-2007
Source: Hee, K.H., Loh, C.S., Yeoh, H.H. (2007-12). Early in vitro flowering and seed production in culture in Dendrobium Chao Praya Smile (Orchidaceae). Plant Cell Reports 26 (12) : 2055-2062. ScholarBank@NUS Repository.
Abstract: Plantlets of Dendrobium Chao Praya Smile maintained in vitro were induced to flower, which produced viable seeds within about 11 months. A two-layer (Gelrite-solidified layer topped with a layer of liquid medium of the same volume and composition) culture system containing benzyladenine (BA) at 11.1 μM induced the highest percent of flowering (45%) in plantlets within 6 months from germination. The percentage of inflorescence induction was increased to 72% by pre-selecting morphologically normal seedlings prior to two-layer culture. Plantlets in culture produced both complete (developmentally normal but smaller than flowers of field grown plants) and incomplete flowers. Pollen and female reproductive organs of in vitro-developed complete flowers were morphologically and anatomically similar to flowers of field grown plants. In addition, 65% of the pollen grains derived from in vitro-developed flower were tetrad suggesting that regular meiosis occurred during microsporogenesis. The percentage of germination of pollen grains derived from in vitro-developed flowers and flowers of field grown plants, incubated on modified Knops' medium for 8 days, were 18.2 and 52.8%, respectively. Despite a lower percentage of germination of the pollen grains derived from in vitro-developed flowers, flowers induced in culture could be self-pollinated and developed seedpods with viable seeds. Nearly 90% of these seeds developed into protocorms on germination in vitro. These seedlings were grown in culture and induced to flower in vitro again using the same procedure. © 2007 Springer-Verlag.
Source Title: Plant Cell Reports
ISSN: 07217714
DOI: 10.1007/s00299-007-0421-9
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Feb 26, 2018


checked on Feb 26, 2018

Page view(s)

checked on Feb 25, 2018

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.