Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.aquaculture.2005.01.031
Title: Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus)
Authors: Chen, S.-L.
Ren, G.-C.
Sha, Z.-X.
Hong, Y. 
Keywords: Embryonic cell line
Karyotype
Scophthalmus maximus
Turbot
Issue Date: 12-Sep-2005
Citation: Chen, S.-L., Ren, G.-C., Sha, Z.-X., Hong, Y. (2005-09-12). Development and characterization of a continuous embryonic cell line from turbot (Scophthalmus maximus). Aquaculture 249 (1-4) : 63-68. ScholarBank@NUS Repository. https://doi.org/10.1016/j.aquaculture.2005.01.031
Abstract: A continuous cell line, TEC (turbot embryonic cell line), was established from embryos at the gastrula stage of a cultured marine fish, turbot (Scophthalmus maximus), and has been cultured for more than 200 days with more than 60 passages. The TEC cells were cultured in DMEM medium supplemented with antibiotics, fetal bovine serum (FBS), sea perch serum (SPS), and basic fibroblast growth factor (bFGF). The cells were small and round, and grew actively and stably in culture. Effect of temperature, fetal bovine serum (FBS) concentration, and bFGF on the growth of TEC cells was examined. The cells grew well in the temperature range of 24-30°C, but have a reduced growth rate at temperatures below 18°C. The growth rate of TEC cells in medium containing 15% FBS was higher than that in medium containing 7.5% FBS. Addition of bFGF to the medium significantly increased the growth rate of TEC cells. Chromosome analysis revealed that TEC cells have a normal diploid karyotype with 2n = 4m + 12st + 28t. High survival rate has been obtained after cryopreservation of cell cultures. © 2005 Elsevier B.V. All rights reserved.
Source Title: Aquaculture
URI: http://scholarbank.nus.edu.sg/handle/10635/100436
ISSN: 00448486
DOI: 10.1016/j.aquaculture.2005.01.031
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

39
checked on Jul 17, 2018

WEB OF SCIENCETM
Citations

37
checked on Jul 17, 2018

Page view(s)

38
checked on Jun 8, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.