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|Title:||Crystal structure of full-length Mycobacterium tuberculosis H37Rv glycogen branching enzyme: Insights of N-terminal β-sandwich in substrate specificity and enzymatic activity|
|Citation:||Pal, K., Kumar, S., Sharma, S., Garg, S.K., Alam, M.S., Xu, H.E., Agrawal, P., Swaminathan, K. (2010-07-02). Crystal structure of full-length Mycobacterium tuberculosis H37Rv glycogen branching enzyme: Insights of N-terminal β-sandwich in substrate specificity and enzymatic activity. Journal of Biological Chemistry 285 (27) : 20897-20903. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M110.121707|
|Abstract:||The open reading frame Rv1326c of Mycobacterium tuberculosis (Mtb) H37Rv encodes for an α-1,4-glucan branching enzyme (MtbGlgB, EC 18.104.22.168, Uniprot entry Q10625). This enzyme belongs to glycoside hydrolase (GH) family 13 and catalyzes the branching of a linear glucose chain during glycogenesis by cleaving a 1→4 bond and making a new 1→6 bond. Here, we show the crystal structure of full-length MtbGlgB (MtbGlgBWT) at 2.33-Å resolution. MtbGlgBWT contains four domains: N1 β-sandwich, N2 β-sandwich, a central (β/α)8 domain that houses the catalytic site, and a C-terminal β-sandwich. We have assayed the amylase activity with amylose and starch as substrates and the glycogen branching activity using amylose as a substrate for MtbGlgBWT and the N1 domain-deleted (the first 108 residues deleted) MtbΔ108GlgB protein. The N1 β-sandwich, which is formed by the first 105 amino acids and superimposes well with the N2 β-sandwich, is shown to have an influence in substrate binding in the amylase assay. Also, we have checked and shown that several GH13 family inhibitors are ineffective against MtbGlgBWT and MtbΔ108GlgB. We propose a twostep reaction mechanism, for the amylase activity (1→4 bond breakage) and isomerization (1→6 bond formation), which occurs in the same catalytic pocket. The structural and functional properties of MtbGlgB and MtbΔ108GlgB are compared with those of the N-terminal 112-amino acid-deleted Escherichia coli GlgB (ECΔ112GlgB). © 2010 by The American Society for Biochemistry and Molecular Biology, Inc.|
|Source Title:||Journal of Biological Chemistry|
|Appears in Collections:||Staff Publications|
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