Please use this identifier to cite or link to this item: https://doi.org/10.1186/1471-2164-12-3
Title: Comprehensive profiling of zebrafish hepatic proximal promoter CpG island methylation and its modification during chemical carcinogenesis
Authors: Mirbahai, L.
Williams, T.D.
Zhan, H. 
Gong, Z. 
Chipman, J.K.
Issue Date: 4-Jan-2011
Citation: Mirbahai, L., Williams, T.D., Zhan, H., Gong, Z., Chipman, J.K. (2011-01-04). Comprehensive profiling of zebrafish hepatic proximal promoter CpG island methylation and its modification during chemical carcinogenesis. BMC Genomics 12 : -. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-2164-12-3
Abstract: Background: DNA methylation is an epigenetic mechanism associated with regulation of gene expression and it is modulated during chemical carcinogenesis. The zebrafish is increasingly employed as a human disease model; however there is a lack of information on DNA methylation in zebrafish and during fish tumorigenesis.Results: A novel CpG island tiling array containing 44,000 probes, in combination with immunoprecipitation of methylated DNA, was used to achieve the first comprehensive methylation profiling of normal adult zebrafish liver. DNA methylation alterations were detected in zebrafish liver tumors induced by the environmental carcinogen 7, 12-dimethylbenz(a)anthracene. Genes significantly hypomethylated in tumors were associated particularly with proliferation, glycolysis, transcription, cell cycle, apoptosis, growth and metastasis. Hypermethylated genes included those associated with anti-angiogenesis and cellular adhesion. Of 49 genes that were altered in expression within tumors, and which also had appropriate CpG islands and were co-represented on the tiling array, approximately 45% showed significant changes in both gene expression and methylation.Conclusion: The functional pathways containing differentially methylated genes in zebrafish hepatocellular carcinoma have also been reported to be aberrantly methylated during tumorigenesis in humans. These findings increase the confidence in the use of zebrafish as a model for human cancer in addition to providing the first comprehensive mapping of DNA methylation in the normal adult zebrafish liver. © 2011 Mirbahai et al; licensee BioMed Central Ltd.
Source Title: BMC Genomics
URI: http://scholarbank.nus.edu.sg/handle/10635/100314
ISSN: 14712164
DOI: 10.1186/1471-2164-12-3
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
2011-comprehensive_profiling _zebrafish-published.pdf3.11 MBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

36
checked on Oct 12, 2018

WEB OF SCIENCETM
Citations

29
checked on Oct 3, 2018

Page view(s)

39
checked on Oct 19, 2018

Download(s)

3
checked on Oct 19, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.