Please use this identifier to cite or link to this item: https://doi.org/10.1073/pnas.0504666102
Title: Cloning of a human parvovirus by molecular screening of respiratory tract samples
Authors: Allander, T.
Tammi, M.T. 
Eriksson, M.
Bjerkner, A.
Tiveljung-Lindell, A.
Andersson, B.
Keywords: Bioinformatics
Nucleotide sequencing
Respiratory tract infection
Virus
Issue Date: 6-Sep-2005
Source: Allander, T., Tammi, M.T., Eriksson, M., Bjerkner, A., Tiveljung-Lindell, A., Andersson, B. (2005-09-06). Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proceedings of the National Academy of Sciences of the United States of America 102 (36) : 12891-12896. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.0504666102
Abstract: The identification of new virus species is a key issue for the study of infectious disease but is technically very difficult. We developed a system for large-scale molecular virus screening of clinical samples based on host DNA depletion, random PCR amplification, large-scale sequencing, and bioinformatics. The technology was applied to pooled human respiratory tract samples. The first experiments detected seven human virus species without the use of any specific reagent. Among the detected viruses were one coronavirus and one parvovirus, both of which were at that time uncharacterized. The parvovirus, provisionally named human bocavirus, was in a retrospective clinical study detected in 17 additional patients and associated with lower respiratory tract infections in children. The molecular virus screening procedure provides a general culture-independent solution to the problem of detecting unknown virus species in single or pooled samples. We suggest that a systematic exploration of the viruses that infect humans, "the human virome," can be initiated. © 2005 by The National Academy of Sciences of the USA.
Source Title: Proceedings of the National Academy of Sciences of the United States of America
URI: http://scholarbank.nus.edu.sg/handle/10635/100282
ISSN: 00278424
DOI: 10.1073/pnas.0504666102
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

1,004
checked on Feb 15, 2018

WEB OF SCIENCETM
Citations

909
checked on Jan 30, 2018

Page view(s)

15
checked on Feb 19, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.