Please use this identifier to cite or link to this item:
|Title:||Biochemical and structural analysis of Gox2181, a new member of the SDR superfamily from Gluconobacter oxydans|
Adam Yuan, Y.
|Citation:||Liu, X., Yuan, Z., Adam Yuan, Y., Lin, J., Wei, D. (2011-11-18). Biochemical and structural analysis of Gox2181, a new member of the SDR superfamily from Gluconobacter oxydans. Biochemical and Biophysical Research Communications 415 (2) : 410-415. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bbrc.2011.10.083|
|Abstract:||Gluconobacter oxydans enable to oxidize sugars and polyols incompletely to corresponding materials with potential industrial applications, containing around 75 putative dehydrogenases. One of these putative dehydrogenases, Gox2181, was cloned and expressed in Escherichia coli BL21 (DE3), and its X-ray crystal structure was determined to a resolution of 1.8å. Gox2181 formed a homo-tetramer in the crystal that was coincident with the apparent molecular mass determined in the solution. Gox2181 displayed α/β-folding patterns, the conserved catalytic tetrad of Asn119-Ser147-Tyr162-Lys166, and the NAD-binding pocket, which aligned well with the 'classical' type of short-chain dehydrogenase/reductase (SDR) enzymes. Gox2181 was denoted SDR51C based on the SDR nomenclature system. The purified recombinant Gox2181 was demonstrated to be NAD(H)-dependent and active towards a wide range of substrates, including sugar alcohols, secondary alcohols, ketones, and ketoses. Among the substrates tested, Gox2181 displayed preference for secondary hydroxyl or carbonyl groups, showing low K m values with d-arabitol and butanedione. © 2011 Elsevier Inc..|
|Source Title:||Biochemical and Biophysical Research Communications|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 14, 2018
WEB OF SCIENCETM
checked on Jun 19, 2018
checked on Jul 13, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.