Tan, L.-P.Chen, G.Y.J.Yao, S.Q.CHEMISTRYBIOLOGICAL SCIENCES2014-06-232014-06-232004-12-06Tan, L.-P., Chen, G.Y.J., Yao, S.Q. (2004-12-06). Expanding the scope of site-specific protein biotinylation strategies using small molecules. Bioorganic and Medicinal Chemistry Letters 14 (23) : 5735-5738. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bmcl.2004.09.0560960894Xhttps://scholarbank.nus.edu.sg/handle/10635/76151We present a new approach to site-specifically biotinylate protein in a cell-free protein synthesis system with puromycin-containing small molecules. With this new method, biotinylated proteins were generated from the DNA templates in a matter of hours, making it useful for protein microarray generation. We also validated that the method is compatible with other high-throughput cloning/proteomics methods. © 2004 Elsevier Ltd. All rights reserved.BiotinylationCell-free protein expressionMicroarrayProteinPuromycinArticle000224953300003