Tan, D.C.W.Kini, R.ManjunathaJois, S.D.S.Lim, D.K.F.Xin, L.Ge, R.BIOLOGICAL SCIENCESPHARMACY2014-10-272014-10-272001-04-13Tan, D.C.W., Kini, R.Manjunatha, Jois, S.D.S., Lim, D.K.F., Xin, L., Ge, R. (2001-04-13). A small peptide derived from Flt-1 (VEGFR-1) functions as an angiogenic inhibitor. FEBS Letters 494 (3) : 150-156. ScholarBank@NUS Repository. https://doi.org/10.1016/S0014-5793(01)02314-600145793https://scholarbank.nus.edu.sg/handle/10635/99968Vascular endothelial growth factor (VEGF) is an angiogenic stimulator which functions through two endothelial specific tyrosine kinase receptors, Flt-1 and Flk-1. In this work, we show that an 11-amino acid peptide derived from the second immunoglobulin-like domain of Flt-1 functions as an angiogenic inhibitor in chick chorioallantoic membrane and inhibited VEGF-induced vascular permeability in Miles' assay without binding to VEGF directly. Circular dichroism and nuclear magnetic resonance analyses indicate that this peptide forms a stable extended structure in solution, presumably β-sheet structure and is most likely existing as a dimer. Our results suggest that this small peptide functions as an angiogenic inhibitor by inhibiting VEGF function through a non-VEGF binding mechanism. © 2001 Federation of European Biochemical Societies.AngiogenesisChick chorioallantoic membraneFlt-1(VEGFR-1)Miles' assayVascular endothelial growth factor (vascular permeability factor)Article000168223800004