Sabanayagam, CharumathiLye, Weng KitJanuszewski, AndrzejAbdul, Riswana Banu Binte MohammedCheung, Gemmy Chui MingKumari, NeelamWong, Tien YCheng, Ching-YuLamoureux, EcosseDUKE-NUS MEDICAL SCHOOL2020-06-052020-06-052017-05-01Sabanayagam, Charumathi, Lye, Weng Kit, Januszewski, Andrzej, Abdul, Riswana Banu Binte Mohammed, Cheung, Gemmy Chui Ming, Kumari, Neelam, Wong, Tien Y, Cheng, Ching-Yu, Lamoureux, Ecosse (2017-05-01). Urinary Isoprostane Levels and Age-Related Macular Degeneration. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 58 (5) : 2538-2543. ScholarBank@NUS Repository. https://doi.org/10.1167/iovs.16-212630146-04041552-5783https://scholarbank.nus.edu.sg/handle/10635/169456© 2017 The Authors. PURPOSE. Oxidative stress, characterized by an excessive production of reactive oxygen intermediates has been suggested to play a role in the pathogenesis of age-related macular degeneration (AMD). We examined the association of urinary F2-isoprostanes (F2-IsoPs), a marker of lipid peroxidation and the most reliable marker of oxidative damage with AMD. METHODS. We included 238 adults with AMD and 390 age-and sex-matched controls without AMD who participated in a population-based cross-sectional study in Singapore (Singapore Chinese Eye Study, 2009-2011). AMD was graded from retinal photographs using the Wisconsin Age-Related Maculopathy Grading System. Urinary-free F2-IsoPs (pmol/mmol of creatinine) were measured by gas chromatography mass spectrometry (GC-MS). The association between F2-IsoPs and AMD was examined using unconditional logistic regression models adjusted for potential confounders including smoking, body mass index (BMI), blood pressure, total and high-density lipoprotein cholesterol, and history of cardiovascular disease. RESULTS. Higher levels of F2-IsoPs were associated with AMD independent of potential confounders. Compared to quartile 1 (Q1) of F2-IsoPs, the multivariable odds ratio (95% confidence interval) of AMD in quartiles 2, 3, and 4 were 2.05 (1.26-3.32), 1.80 (1.10-2.94), and 1.76 (1.06-2.94), respectively. In subgroup analyses comparing Q4 to Q1, this association was stronger in women, those with BMI less than 25 kg/m2 and those with hypertension, but no significant interaction was found (P interaction > 0.1 for each strata). CONCLUSIONS. Higher levels of urinary F2-IsoPs levels were associated with AMD independent of potential confounders in Chinese adults.enScience & TechnologyLife Sciences & BiomedicineOphthalmologyage-related macular degenerationAsiansisoprostaneoxidative stressC-REACTIVE PROTEINSTRESS IN-VIVOHUMAN ATHEROSCLEROTIC LESIONSCORONARY-ARTERY-DISEASEOXIDATIVE STRESSLIPID-PEROXIDATIONCARDIOVASCULAR-DISEASEMASS-SPECTROMETRYOXYGEN-TENSIONOXIDANT STRESSArticle2020-06-03