Kou, S.S.Waller, L.Barbastathis, G.Sheppard, C.J.R.BIOENGINEERING2014-06-172014-06-172010-02-01Kou, S.S., Waller, L., Barbastathis, G., Sheppard, C.J.R. (2010-02-01). Transport-of-intensity approach to differential interference contrast (TI-DIC) microscopy for quantitative phase imaging. Optics Letters 35 (3) : 447-449. ScholarBank@NUS Repository. https://doi.org/10.1364/OL.35.00044701469592https://scholarbank.nus.edu.sg/handle/10635/67335Differential interference contrast (DIC) microscopy is an inherently qualitative phase-imaging technique. What is obtained is an image with mixed phase-gradient and amplitude information rather than a true linear mapping of actual optical path length (OPL) differences. Here we investigate an approach that combines the transport-of-intensity equation (TIE) with DIC microscopy, thus improving direct visual observation. There is little hardware modification and the computation is noniterative. Numerically solving for the propagation of light in a series of through-focus DIC images allows linear phase information in a single slice to be completely determined and restored from DIC intensity values © 2010 Optical Society of America.Article000274196100060