Please use this identifier to cite or link to this item: https://doi.org/10.1007/s12015-009-9092-y
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dc.titleA three dimensional anchorage independent In Vitro system for the prolonged growth of embryoid bodies to study cancer cell behaviour and anticancer agents
dc.contributor.authorFong, C.-Y.
dc.contributor.authorChak, L.-L.
dc.contributor.authorSubramanian, A.
dc.contributor.authorTan, J.-H.
dc.contributor.authorBiswas, A.
dc.contributor.authorGauthaman, K.
dc.contributor.authorChoolani, M.
dc.contributor.authorChan, W.-K.
dc.contributor.authorBongso, A.
dc.date.accessioned2014-10-27T08:20:40Z
dc.date.available2014-10-27T08:20:40Z
dc.date.issued2010-01
dc.identifier.citationFong, C.-Y., Chak, L.-L., Subramanian, A., Tan, J.-H., Biswas, A., Gauthaman, K., Choolani, M., Chan, W.-K., Bongso, A. (2010-01). A three dimensional anchorage independent In Vitro system for the prolonged growth of embryoid bodies to study cancer cell behaviour and anticancer agents. Stem Cell Reviews and Reports 5 (4) : 410-419. ScholarBank@NUS Repository. https://doi.org/10.1007/s12015-009-9092-y
dc.identifier.issn15508943
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/99978
dc.description.abstractWe describe a three dimensional (3D) anchorage independent in vitro protocol for the prolonged growth of human embryoid bodies (EBs) up to 90 days. We grew hESCs (46XX) in methylcellulose (MC) in motion culture in the presence of EB medium (EB), EB medium with Matrigel (EB + MAT), bulk culture medium (BCM), and BCM medium with Matrigel (BCM + MAT). All four experimental groups produced embryoid bodies (EBs) which with prolonged growth to 90 days acquired blood vessels and tissues from all three germ layers. Based on histology, microarray gene expression profiles and the definition for experimental teratomas, we could classify the EBs into early EBs, mature EBs and teratomas. The EB + MAT group produced the highest number of teratomas and their microarray data suggested the presence of inductive microenvironment niches and activation of pathways for self-organization, morphogenesis and growth. When we microinjected hepatocarcinoma-Green Fluorescent Protein cells (HepG2-GFP) (46XY) into the teratomas, after 10 days the HepG2-GFP cells had grown inside the teratoma as confirmed by confocal microscopy and SRY gene analysis. This 3D-MC-(EB + MAT) in vitro system requires few cells to produce many teratomas, can be used to test pluripotency of potential human embryonic and induced pluripotent stem cell lines (hESC, hiPSC), and is an experimental humanized platform to study cancer cell behavior. © Humana Press 2009.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s12015-009-9092-y
dc.sourceScopus
dc.subjectEmbryoid bodies
dc.subjectHistology
dc.subjectHuman embryonic stem cells
dc.subjectIn vitro teratoma assay
dc.subjectMicroarray
dc.subjectMicroinjection
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1007/s12015-009-9092-y
dc.description.sourcetitleStem Cell Reviews and Reports
dc.description.volume5
dc.description.issue4
dc.description.page410-419
dc.identifier.isiut000273470400012
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