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|Title:||A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases||Authors:||Lim, S.
|Issue Date:||1-Jul-2013||Citation:||Lim, S., Wang, Y., Yu, X., Huang, Y., Featherstone, M.S., Sampath, K. (2013-07-01). A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases. Genome Biology 14 (7) : -. ScholarBank@NUS Repository. https://doi.org/10.1186/gb-2013-14-7-r69||Abstract:||Precise and effective genome-editing tools are essential for functional genomics and gene therapy. Targeting nucleases have been successfully used to edit genomes. However, whole-locus or element-specific deletions abolishing transcript expression have not previously been reported. Here, we show heritable targeting of locus-specific deletions in the zebrafish nodal-related genes squint (sqt) and cyclops (cyc). Our strategy of heritable chromosomal editing can be used for disease modeling, analyzing gene clusters, regulatory regions, and determining the functions of non-coding RNAs in genomes. © 2013 Lim et al.; licensee BioMed Central Ltd.||Source Title:||Genome Biology||URI:||http://scholarbank.nus.edu.sg/handle/10635/99964||ISSN:||1474760X||DOI:||10.1186/gb-2013-14-7-r69|
|Appears in Collections:||Staff Publications|
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