Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/99923
DC FieldValue
dc.titleA novel serine protease inhibitor acts as an immunomodulatory switch while maintaining homeostasis
dc.contributor.authorJiang, N.
dc.contributor.authorThangamani, S.
dc.contributor.authorChor, C.F.
dc.contributor.authorWang, S.Y.
dc.contributor.authorWinarsih, I.
dc.contributor.authorDu, R.J.
dc.contributor.authorSivaraman, J.
dc.contributor.authorHo, B.
dc.contributor.authorDing, J.L.
dc.date.accessioned2014-10-27T08:20:04Z
dc.date.available2014-10-27T08:20:04Z
dc.date.issued2009-07
dc.identifier.citationJiang, N., Thangamani, S., Chor, C.F., Wang, S.Y., Winarsih, I., Du, R.J., Sivaraman, J., Ho, B., Ding, J.L. (2009-07). A novel serine protease inhibitor acts as an immunomodulatory switch while maintaining homeostasis. Journal of Innate Immunity 1 (5) : 465-479. ScholarBank@NUS Repository.
dc.identifier.issn1662811X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/99923
dc.description.abstractSerine protease cascades boost immune responses while maintaining homeostasis. These crucial actions are intricately regulated by cognate serine protease inhibitors. However, the mechanism underlying such a dynamic immunomodulation during acute phase infection remains obscure, particularly where the pathogen's serine protease adds a new challenge to the host. Here, we found that infection of horseshoe crab, Carcinoscorpius rotundicauda, induced reciprocal profiles of CrSPI (serine protease inhibitor) and CrFurin (serine protease) with respect to their transcription and protein activities. Using recombinant rCrSPI, we explored its inhibitory activity against various microbial proteases and found it most efficacious against a model serine protease, subtilisin A. rCrSPI inhibited subtilisin at Ki 10-9M with a molar ratio of 1 rCrSPI:2 subtilisin. The rCrSPI also inhibited plasma CrFurin, suppressed subtilisin-mediated activation of prophenoloxidase (PPO) and interacted with complement C3. Taken together, CrSPI acts as a key immunomodulatory 'on-off' switch in a 2-way regulation of serine protease microbial subtilisin and host serine proteases (CrFurin and CrC3), thereby controlling immune responses involving the complements and the PPO-mediated antimicrobial activities, while maintaining homeostasis. Copyright © 2009 S. Karger AG.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1159/000209224
dc.sourceScopus
dc.subjectHomeostasis
dc.subjectImmunomodulation
dc.subjectKinetic profiles
dc.subjectProphenoloxidase pathway
dc.subjectSerine protease inhibitor
dc.subjectSerine proteases
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.sourcetitleJournal of Innate Immunity
dc.description.volume1
dc.description.issue5
dc.description.page465-479
dc.identifier.isiut000268738000009
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.