Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0303-7207(98)00191-9
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dc.titleA novel piscine vitellogenin gene: Structural and functional analyses of estrogen-inducible promoter
dc.contributor.authorTeo, B.Y.
dc.contributor.authorTan, N.S.
dc.contributor.authorLim, E.H.
dc.contributor.authorLam, T.J.
dc.contributor.authorDing, J.L.
dc.date.accessioned2014-10-27T08:20:03Z
dc.date.available2014-10-27T08:20:03Z
dc.date.issued1998-11-25
dc.identifier.citationTeo, B.Y., Tan, N.S., Lim, E.H., Lam, T.J., Ding, J.L. (1998-11-25). A novel piscine vitellogenin gene: Structural and functional analyses of estrogen-inducible promoter. Molecular and Cellular Endocrinology 146 (1-2) : 103-120. ScholarBank@NUS Repository. https://doi.org/10.1016/S0303-7207(98)00191-9
dc.identifier.issn03037207
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/99921
dc.description.abstractThe Oreochromis aureus vitellogenin, OaVtg, gene spans 9 kb and contains 34 exons. Its transcription start site is located 15 bp upstream of the translational start codon. Although the OaVtg promoter has a nonconsensus TATA, transient transfection assay showed that this promoter is capable of driving basal transcription. Two imperfect estrogen response elements: ERE(p) (proximal) and ERE(d) (distal) are located in the promoter at - 532 and - 1352, respectively. In competition gel mobility-shift assays, only ERE(p) exhibited specific binding of the recombinant estrogen receptor protein, GST- C/D OaER. Another imperfect ERE (ERE(exon2)) was detected within exon 2 of the OaVtg gene. This is a novel finding for a vitellogenin (Vtg) gene. ERE(exon2) similarly showed specific recognition of GST-C/D OaER. Both EREp and ERE(exon2) showed comparable binding affinities as consensus ERE. In transient transfections, the OaVtg promoter, ERE(p) and ERE(d) elicited significant increase in estrogen-dependent synthesis of CAT protein. Hence, we propose that the non-consensus OaVtg EREs contribute to the estrogen- dependent regulation of the OaVtg gene in vivo.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0303-7207(98)00191-9
dc.sourceScopus
dc.subjectDNA-binding
dc.subjectImperfect estrogen response elements
dc.subjectOreochromis aureus
dc.subjectPromoter
dc.subjectRecombinant estrogen receptor
dc.subjectVitellogenin gene
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/S0303-7207(98)00191-9
dc.description.sourcetitleMolecular and Cellular Endocrinology
dc.description.volume146
dc.description.issue1-2
dc.description.page103-120
dc.description.codenMCEND
dc.identifier.isiut000077924200012
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