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https://doi.org/10.1042/BSR20130098
| Title: | A comparison and optimization of methods and factors affecting the transformation of Escherichia coli | Authors: | Chan, W.-T. Verma, C.S. Lane, D.P. Gan, S.K.-E. |
Keywords: | CaCl2 method Competent bacteria DMSO method Hanahan's method Heat shock |
Issue Date: | 2013 | Citation: | Chan, W.-T., Verma, C.S., Lane, D.P., Gan, S.K.-E. (2013). A comparison and optimization of methods and factors affecting the transformation of Escherichia coli. Bioscience Reports 33 (6) : -. ScholarBank@NUS Repository. https://doi.org/10.1042/BSR20130098 | Abstract: | DNA manipulation routinely requires competent bacteria that can be made using one of numerous methods. To determine the best methods, we compared four commonly used chemical methods (DMSO, MgCl2-CaCl2, CaCl2 and Hanahan's methods) on frequently used Escherichia coli (E. coli) strains: DH5α, XL-1 Blue, SCS110, JM109, TOP10 and BL21-(DE3)-PLysS. Hanahan's method was found to be most effective for DH5α, XL-1 Blue and JM109 strains (P | Source Title: | Bioscience Reports | URI: | http://scholarbank.nus.edu.sg/handle/10635/99811 | ISSN: | 01448463 | DOI: | 10.1042/BSR20130098 |
| Appears in Collections: | Staff Publications |
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