Please use this identifier to cite or link to this item: https://doi.org/10.1042/BSR20130098
Title: A comparison and optimization of methods and factors affecting the transformation of Escherichia coli
Authors: Chan, W.-T.
Verma, C.S. 
Lane, D.P.
Gan, S.K.-E.
Keywords: CaCl2 method
Competent bacteria
DMSO method
Hanahan's method
Heat shock
Issue Date: 2013
Citation: Chan, W.-T., Verma, C.S., Lane, D.P., Gan, S.K.-E. (2013). A comparison and optimization of methods and factors affecting the transformation of Escherichia coli. Bioscience Reports 33 (6) : -. ScholarBank@NUS Repository. https://doi.org/10.1042/BSR20130098
Abstract: DNA manipulation routinely requires competent bacteria that can be made using one of numerous methods. To determine the best methods, we compared four commonly used chemical methods (DMSO, MgCl2-CaCl2, CaCl2 and Hanahan's methods) on frequently used Escherichia coli (E. coli) strains: DH5α, XL-1 Blue, SCS110, JM109, TOP10 and BL21-(DE3)-PLysS. Hanahan's method was found to be most effective for DH5α, XL-1 Blue and JM109 strains (P
Source Title: Bioscience Reports
URI: http://scholarbank.nus.edu.sg/handle/10635/99811
ISSN: 01448463
DOI: 10.1042/BSR20130098
Appears in Collections:Staff Publications

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