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https://doi.org/10.1007/s002990050207
Title: | High frequency adventitious shoot regeneration from excised leaves of Paulownia spp. cultured in vitro | Authors: | Dimps Rao, C. Goh, C.-J. Kumar, P.P. |
Issue Date: | 1996 | Citation: | Dimps Rao, C.,Goh, C.-J.,Kumar, P.P. (1996). High frequency adventitious shoot regeneration from excised leaves of Paulownia spp. cultured in vitro. Plant Cell Reports 16 (3-4) : 204-209. ScholarBank@NUS Repository. https://doi.org/10.1007/s002990050207 | Abstract: | High frequency, direct regeneration of shoots was induced in leaf cultures of Paulownia tomentosa, P. fortunei x P. tomentosa and P. kawakamii. The optimum culture medium for the leaf explants derived from shoot cultures was Murashige-Skoog (MS) medium supplemented with 10 μM indole-3-acetic acid and 50 μM benzyladenine. Up to 40 shoots were obtained over a 4 month culture period from each leaf explant. Rooting occurred spontaneously in the shoots that were about 1 cm tall when subcultured on phytohormone-free MS medium. The plantlets could be transplanted successfully. Some of the transplanted P. tomentosa plantlets flowered in the greenhouse one year after transplanting. The protocol is suitable not only for rapid multiplication of the various species of Paulownia, but also for analytical studies associated with adventitious shoot regeneration. | Source Title: | Plant Cell Reports | URI: | http://scholarbank.nus.edu.sg/handle/10635/99684 | ISSN: | 07217714 | DOI: | 10.1007/s002990050207 |
Appears in Collections: | Staff Publications |
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