Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF02632024
DC FieldValue
dc.titleAmmonium and nitrate uptake and nitrate reductase activity of photoautotrophic callus cultures of the fern Platycerium coronarium (Koenig) DESV
dc.contributor.authorKwa, S.-H.
dc.contributor.authorWee, Y.-C.
dc.contributor.authorKumar, P.P.
dc.date.accessioned2014-10-27T07:02:44Z
dc.date.available2014-10-27T07:02:44Z
dc.date.issued1995-10
dc.identifier.citationKwa, S.-H.,Wee, Y.-C.,Kumar, P.P. (1995-10). Ammonium and nitrate uptake and nitrate reductase activity of photoautotrophic callus cultures of the fern Platycerium coronarium (Koenig) DESV. In Vitro Cellular & Developmental Biology - Plant 31 (4) : 211-214. ScholarBank@NUS Repository. <a href="https://doi.org/10.1007/BF02632024" target="_blank">https://doi.org/10.1007/BF02632024</a>
dc.identifier.issn10545476
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/99644
dc.description.abstractThe uptake of nitrate and ammonium by callus of Platycerium coronarium from the culture medium was examined. Nitrate reductase activity of photoautotrophic callus cultures under CO2 enrichment was significantly lower compared to the cultures without CO2 enrichment, but higher than that of heterotrophic callus cultured on medium with 2% (wt/vol) sucrose. When sucrose concentration of the heterotrophic culture was lowered to 0.2%, nitrate reductase activity increased. The level of nitrate reductase activity increased by about 25% in the heterotrophic callus with an increase in 2,4-D from 2 μM to 10 μM, despite a decline in fresh weight gain. However, photoautotrophic cultures with 1% CO2 enrichment showed 20% decline in nitrate reductase activity and 45% decline in fresh weight gain with a similar increase in 2,4-D level. The rate of uptake of nitrate from the culture medium was unrelated to the level of nitrate reductase activity in the callus. For photoautotrophic callus under CO2 enrichment, the presence of 1% (vol/vol) CO2 generally resulted in the highest rate of nitrate uptake. The rate of uptake of ammonium was higher for callus cultured on 2 μM 2,4-D compared to that on 10 μM 2,4-D. © 1995 Society for In Vitro Biology.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/BF02632024
dc.sourceScopus
dc.subjectCO2 enrichment
dc.subjectnitrate reductase activity
dc.subjectphotoautotrophic callus
dc.subjectPlatycerium coronarium
dc.subjectstag's horn fern
dc.typeArticle
dc.contributor.departmentBOTANY
dc.description.doi10.1007/BF02632024
dc.description.sourcetitleIn Vitro Cellular & Developmental Biology - Plant
dc.description.volume31
dc.description.issue4
dc.description.page211-214
dc.description.codenIVCPE
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

2
checked on Jan 23, 2022

Page view(s)

246
checked on Jan 20, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.