Please use this identifier to cite or link to this item: https://doi.org/10.1021/nn3038258
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dc.titleSingle-molecule DNA analysis reveals that yeast Hop1 protein promotes DNA folding and synapsis: Implications for condensation of meiotic chromosomes
dc.contributor.authorKhan, K.
dc.contributor.authorKarthikeyan, U.
dc.contributor.authorLi, Y.
dc.contributor.authorYan, J.
dc.contributor.authorMuniyappa, K.
dc.date.accessioned2014-10-16T09:41:08Z
dc.date.available2014-10-16T09:41:08Z
dc.date.issued2012-12-21
dc.identifier.citationKhan, K., Karthikeyan, U., Li, Y., Yan, J., Muniyappa, K. (2012-12-21). Single-molecule DNA analysis reveals that yeast Hop1 protein promotes DNA folding and synapsis: Implications for condensation of meiotic chromosomes. ACS Nano 6 (12) : 10658-10666. ScholarBank@NUS Repository. https://doi.org/10.1021/nn3038258
dc.identifier.issn19360851
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/97940
dc.description.abstractDuring meiosis, long-range interaction between homologous chromosomes is thought to be crucial for homology recognition, exchange of DNA strands, and production of normal haploid gametes. However, little is known about the identity of the proteins involved and the actual molecular mechanism(s) by which chromosomes recognize and recombine with their appropriate homologous partners. Single-molecule analyses have the potential to provide insights into our understanding of this fascinating and long-standing question. Using atomic force microscopy and magnetic tweezers techniques, we discovered that Hop1 protein, a key structural component of Saccharomyces cerevisiae synaptonemal complex, exhibits the ability to bridge noncontiguous DNA segments into intramolecular stem-loop structures in which the DNA segments appear to be fully synapsed within the filamentous protein stems. Additional evidence suggests that Hop1 folds DNA into rigid protein-DNA filaments and higher-order nucleoprotein structures. Importantly, Hop1 promotes robust intra- and intermolecular synapsis between double-stranded DNA molecules, suggesting that juxtaposition of DNA sequences may assist in strand exchange between homologues by recombination-associated proteins. Finally, the evidence from ensemble experiments is consistent with the notion that Hop1 causes rigidification of DNA molecules. These results provide the first direct evidence for long-range protein-mediated DNA-DNA synapsis, independent of crossover recombination, which is presumed to occur during meiotic recombination. © 2012 American Chemical Society.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/nn3038258
dc.sourceScopus
dc.subjectDNA bridging
dc.subjectDNA condensation
dc.subjectHolliday junction
dc.subjectHop1 protein
dc.subjectlong-range DNA interactions
dc.subjectmeiosis
dc.typeArticle
dc.contributor.departmentPHYSICS
dc.description.doi10.1021/nn3038258
dc.description.sourcetitleACS Nano
dc.description.volume6
dc.description.issue12
dc.description.page10658-10666
dc.identifier.isiut000312563600026
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