Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/97384
DC FieldValue
dc.titleNuclear microscopy of single whole cultured cells: Beam damage studies
dc.contributor.authorWatt, F.
dc.contributor.authorThong, P.S.P.
dc.contributor.authorTan, A.H.M.
dc.contributor.authorTang, S.M.
dc.date.accessioned2014-10-16T09:34:37Z
dc.date.available2014-10-16T09:34:37Z
dc.date.issued1997-07
dc.identifier.citationWatt, F.,Thong, P.S.P.,Tan, A.H.M.,Tang, S.M. (1997-07). Nuclear microscopy of single whole cultured cells: Beam damage studies. Nuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms 130 (1-4) : 188-191. ScholarBank@NUS Repository.
dc.identifier.issn0168583X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/97384
dc.description.abstractElemental analysis of individual whole cultured cells using Particle Induced X-ray Emission (PIXE), Scanning Transmission Ion Microscopy (STIM) and Rutherford Backscattering Spectrometry (RBS) can yield quantitative results at the parts per million level. During a continuous scan over a single cell using 2 MeV protons (with a beam current of 100 pA, spot size of 1 μm), elemental loss was monitored and a 60% reduction in hydrogen and oxygen was observed. Concentrations of C, Na, Mg, P, S, Cl, K, Ca, Fe, Cu, and Zn remained constant. The elemental analysis, at the parts per million level, of single whole cultured cells is therefore not limited by beam damage. However, during the irradiation, the size of the cell was observed to shrink by 20%, placing severe restrictions on the imaging and analysis of subcellular structures such as organelles.
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentPHYSICS
dc.description.sourcetitleNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
dc.description.volume130
dc.description.issue1-4
dc.description.page188-191
dc.description.codenNIMBE
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Page view(s)

58
checked on Mar 29, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.