Please use this identifier to cite or link to this item: https://doi.org/10.1002/marc.200900818
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dc.titleInjectable biodegradable polyethylene glycol/ RGD peptide hybrid hydrogels for in vitro chondrogenesis of human mesenchymal stern cellsa
dc.contributor.authorQiong Liu, S.
dc.contributor.authorTian, Q.
dc.contributor.authorWang, L.
dc.contributor.authorHedrick, J.L.
dc.contributor.authorPo Hui, J.H.
dc.contributor.authorYan Yang, Y.
dc.contributor.authorEe, P.L.R.
dc.date.accessioned2014-10-16T09:29:24Z
dc.date.available2014-10-16T09:29:24Z
dc.date.issued2010-07-01
dc.identifier.citationQiong Liu, S., Tian, Q., Wang, L., Hedrick, J.L., Po Hui, J.H., Yan Yang, Y., Ee, P.L.R. (2010-07-01). Injectable biodegradable polyethylene glycol/ RGD peptide hybrid hydrogels for in vitro chondrogenesis of human mesenchymal stern cellsa. Macromolecular Rapid Communications 31 (13) : 1148-1154. ScholarBank@NUS Repository. https://doi.org/10.1002/marc.200900818
dc.identifier.issn10221336
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/96941
dc.description.abstract(Figure Presented) In this study, an injectable and biodegradable poly(ethylene glycol) (PEG)/arginine-glycine-aspartic (RGD) peptide hybrid hydrogel has been synthesized and used as a biomimetic scaffold for encapsulation of human mesenchymal stem cells (hMSCs). Tetrahydroxyl PEG was functionalized with acrylate, and then reacted with thiol-containing peptide (RGD). Gelation occurred within 30 min with the addition of cells and PEG-dithiol via Michael addition. The hydrogels synthesized with a peptide concentration of 1.0-5.0 mM achieved significantly greater cell viability when compared to the hydrogels without the RGD peptide. However, the effect of RGD on chondrogenesis was found to be dose-dependent. Immunohistology studies demonstrated that hMSCs encapsulated in the hydrogel matrix with 1.0 mM RGD and TGF-ß3 showed enhanced positive staining for aggrecan and type II collagen as compared to that with 5.0 mM RGD and unmodified PEG hydrogels. RT-PCR results further revealed that the cells in hydrogels with 1 mM RGD expressed significantly higher levels of type II collagen than those in PEG hydogels without RGD peptide. These findings have demonstrated that the PEG-RGD hydrogels can be a promising scaffold to deliver hMSCs for cartilage repair. © 2010 WILEY-VCH Verlag GmbH & Co. KCaA, Weinheim.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/marc.200900818
dc.sourceScopus
dc.subjectBiomaterials
dc.subjectChondrogenic differentiation
dc.subjectHuman mesenchymal stem cells
dc.subjectHydrogels
dc.subjectInjectable peg hydrogel
dc.subjectRGD
dc.typeArticle
dc.contributor.departmentPHARMACY
dc.contributor.departmentPHYSICS
dc.description.doi10.1002/marc.200900818
dc.description.sourcetitleMacromolecular Rapid Communications
dc.description.volume31
dc.description.issue13
dc.description.page1148-1154
dc.description.codenMRCOE
dc.identifier.isiut000280045400005
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