Please use this identifier to cite or link to this item: https://doi.org/10.1021/la047967r
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dc.titleEncapsulation of DNA by cationic diblock copolymer vesicles
dc.contributor.authorKorobko, A.V.
dc.contributor.authorJesse, W.
dc.contributor.authorVan Der Maarel, J.R.C.
dc.date.accessioned2014-10-16T09:23:35Z
dc.date.available2014-10-16T09:23:35Z
dc.date.issued2005-01-04
dc.identifier.citationKorobko, A.V., Jesse, W., Van Der Maarel, J.R.C. (2005-01-04). Encapsulation of DNA by cationic diblock copolymer vesicles. Langmuir 21 (1) : 34-42. ScholarBank@NUS Repository. https://doi.org/10.1021/la047967r
dc.identifier.issn07437463
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/96451
dc.description.abstractEncapsulation of dsDNA fragments (contour length 54 nm) by the cationic diblock copolymer poly-(butadiene-b-N-methyl-4-vinyl pyridinium) [PBd-b-P4VPQ] has been studied with phase contrast, polarized light, and fluorescence microscopies, as well as scanning electron microscopy. Encapsulation was achieved with a single emulsion technique. For this purpose, an aqueous DNA solution is emulsified in an organic solvent (toluene) and stabilized by the amphiphilic diblock copolymer. The PBd block forms an interfacial brush, whereas the cationic P4VPQ block complexes with DNA. A subsequent change of the quality of the organic solvent results in a collapse of the PBd brush and the formation of a capsule. Inside the capsules, the DNA is compacted as shown by the appearance of birefringent textures under crossed polarizers and the increase in fluorescence intensity of labeled DNA. The capsules can also be dispersed in an aqueous medium to form vesicles, provided they are stabilized with an osmotic agent [poly(ethylene glycol)] in the external phase. It is shown that the DNA is released from the vesicles once the osmotic pressure drops below 105 N/m2 or if the ionic strength of the supporting medium exceeds 0.1 M. The method has also proven to be efficient to encapsulate pUC18 plasmid in submicrometer-sized vesicles, and the general applicability of the method has been demonstrated by the preparation of the charge inverse system: cationic poly(ethylene imine) encapsulated by the anionic diblock poly(styrene-b-acrylic acid).
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/la047967r
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentPHYSICS
dc.description.doi10.1021/la047967r
dc.description.sourcetitleLangmuir
dc.description.volume21
dc.description.issue1
dc.description.page34-42
dc.description.codenLANGD
dc.identifier.isiut000226232700007
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