Please use this identifier to cite or link to this item: https://doi.org/10.1101/gad.1883510
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dc.titleA divalent switch drives H-NS/DNA-binding conformations between stiffening and bridging modes
dc.contributor.authorLiu, Y.
dc.contributor.authorChen, H.
dc.contributor.authorKenney, L.J.
dc.contributor.authorYan, J.
dc.date.accessioned2014-10-16T09:13:49Z
dc.date.available2014-10-16T09:13:49Z
dc.date.issued2010-02-15
dc.identifier.citationLiu, Y., Chen, H., Kenney, L.J., Yan, J. (2010-02-15). A divalent switch drives H-NS/DNA-binding conformations between stiffening and bridging modes. Genes and Development 24 (4) : 339-344. ScholarBank@NUS Repository. https://doi.org/10.1101/gad.1883510
dc.identifier.issn08909369
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/95618
dc.description.abstractHeat-stable nucleoid structuring protein (H-NS) is an abundant prokaryotic protein that plays important roles in organizing chromosomal DNA and gene silencing. Two controversial binding modes were identified. H-NS binding stimulating DNA bridging has become the accepted mechanism, whereas H-NS binding causing DNA stiffening has been largely ignored. Here, we report that both modes exist, and that changes in divalent cations drive a switch between them. The stiffening formis present under physiological conditions, and directly responds to pH and temperature in vitro. Our findings have broad implications and require a reinterpretation of the mechanism by which H-NS regulates genes. © 2010 by Cold Spring Harbor Laboratory Press.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1101/gad.1883510
dc.sourceScopus
dc.subjectAtomic force
dc.subjectGene silencing
dc.subjectHeat-stable nucleoid structuring protein (H-NS)
dc.subjectMagnetic tweezers
dc.subjectMicroscopy
dc.subjectPathogenicity islands
dc.subjectTranscriptional regulation
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentPHYSICS
dc.description.doi10.1101/gad.1883510
dc.description.sourcetitleGenes and Development
dc.description.volume24
dc.description.issue4
dc.description.page339-344
dc.description.codenGEDEE
dc.identifier.isiut000274577100003
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