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https://doi.org/10.1101/gad.1883510
Title: | A divalent switch drives H-NS/DNA-binding conformations between stiffening and bridging modes | Authors: | Liu, Y. Chen, H. Kenney, L.J. Yan, J. |
Keywords: | Atomic force Gene silencing Heat-stable nucleoid structuring protein (H-NS) Magnetic tweezers Microscopy Pathogenicity islands Transcriptional regulation |
Issue Date: | 15-Feb-2010 | Citation: | Liu, Y., Chen, H., Kenney, L.J., Yan, J. (2010-02-15). A divalent switch drives H-NS/DNA-binding conformations between stiffening and bridging modes. Genes and Development 24 (4) : 339-344. ScholarBank@NUS Repository. https://doi.org/10.1101/gad.1883510 | Abstract: | Heat-stable nucleoid structuring protein (H-NS) is an abundant prokaryotic protein that plays important roles in organizing chromosomal DNA and gene silencing. Two controversial binding modes were identified. H-NS binding stimulating DNA bridging has become the accepted mechanism, whereas H-NS binding causing DNA stiffening has been largely ignored. Here, we report that both modes exist, and that changes in divalent cations drive a switch between them. The stiffening formis present under physiological conditions, and directly responds to pH and temperature in vitro. Our findings have broad implications and require a reinterpretation of the mechanism by which H-NS regulates genes. © 2010 by Cold Spring Harbor Laboratory Press. | Source Title: | Genes and Development | URI: | http://scholarbank.nus.edu.sg/handle/10635/95618 | ISSN: | 08909369 | DOI: | 10.1101/gad.1883510 |
Appears in Collections: | Staff Publications |
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