Please use this identifier to cite or link to this item:
https://doi.org/10.1088/1478-3975/7/3/036003
Title: | A coordinated molecular 'fishing' mechanism in heterodimeric kinesin | Authors: | Hou, R. Wang, Z. |
Issue Date: | Sep-2010 | Citation: | Hou, R., Wang, Z. (2010-09). A coordinated molecular 'fishing' mechanism in heterodimeric kinesin. Physical Biology 7 (3) : -. ScholarBank@NUS Repository. https://doi.org/10.1088/1478-3975/7/3/036003 | Abstract: | Kar3 is a kinesin motor that facilitates chromosome segregation during cell division. Unlike many members of the kinesin superfamily, Kar3 forms a heterodimer with non-motor protein Vik1 or Cik1 in vivo. The heterodimers show ATP-driven minus-end directed motility along a microtubule (MT) lattice, and also serve as depolymerase at the MT ends. The molecular mechanisms behind this dual functionality remain mysterious. Here, a molecular mechanical model for the Kar3/Vik1 heterodimer based on structural, kinetic and motility data reveals a long-range chemomechanical transmission mechanism that resembles a familiar fishing tactic. By this molecular 'fishing', ATP-binding to Kar3 dissociates catalytically inactive Vik1 off MT to facilitate minus-end sliding of the dimer on the MT lattice. When the dimer binds the frayed ends of MT, the fishing channels ATP hydrolysis energy into MT deploymerization by a mechanochemical effect. The molecular fishing thus provides a unified mechanistic ground for Kar3's dual functionality. The fishing-promoted depolymerization differs from the depolymerase mechanisms found in homodimeric kinesins. The fishing also enables intermolecular coordination with a chemomechanical coupling feature different from the paradigmatic pattern of homodimeric motors. This study rationalizes some puzzling experimental observation, and suggests new experiments for further elucidation of the fishing mechanism. © 2010 IOP Publishing Ltd. | Source Title: | Physical Biology | URI: | http://scholarbank.nus.edu.sg/handle/10635/95614 | ISSN: | 14783975 | DOI: | 10.1088/1478-3975/7/3/036003 |
Appears in Collections: | Staff Publications |
Show full item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.