Separation of biogenic amines by micellar electrokinetic chromatography

Abstract

The separation by the micellar electrokinetic method of eight biogenic amines was investigated. Optimum separation of the amines: putrescine, histamine, tyramine, cadaverine, phenethylamine, tryptamine, spermidine and spermine was obtained. The method involved extraction and derivatization of the amines with fluoresceine isothiocyanate. The electrolyte system was berate modified with sodium dodecyl sulfate. Separation was achieved in less than 15 min. Detection limit obtained with on a xenon lamp-based fluorescence detector was 10-8 M. The method developed was used for the analysis of biogenic amines in soy sauce.