1,3-Dialkylimidazolium-based room-temperature ionic liquids as background electrolyte and coating material in aqueous capillary electrophoresis

Abstract

The 1-ethyl-3-methylimidazolium (EMIM) cation was found to have constant mobility of 4.5×10-4 cm2 V-1 s-1 over the pH range of 3 to 11. The electroosmotic flow of bare silica capillary was reversed by the covalently bonded room-temperature ionic liquid (RTIL) coating. With run buffer of 5 mM EMIM (pH 8.5), NH4 + in human urine was separated from the K+ matrix and was detected to be 0.37±0.012%. K+, Na+, Li+, Ca2+, Mg2+, and Ba2+ were baseline separated in RTIL-coated capillary with run buffer of 10 mM EMIMOH-acetic acid at pH 5, and the concentration of the above ions in a red wine were detected to be 907, 27.9, 0, 71.0, 83.4 and 31.1 μg/ml, respectively. The RTIL-coated capillary showed stable electroosmotic flow for at least 80 h in the run buffer. © 2002 Elsevier Science B.V. All rights reserved.