Please use this identifier to cite or link to this item:;2-8
Title: Two-coat systems for encapsulation of Spathoglottis plicata (Orchidaceae) seeds and protocorms
Authors: Khor, E. 
Ng, W.-F.
Loh, C.-S. 
Keywords: Artificial seed
Complex coacervation
Spathoglottis plicata
Two-coat systems
Issue Date: 5-Sep-1998
Citation: Khor, E.,Ng, W.-F.,Loh, C.-S. (1998-09-05). Two-coat systems for encapsulation of Spathoglottis plicata (Orchidaceae) seeds and protocorms. Biotechnology and Bioengineering 59 (5) : 635-639. ScholarBank@NUS Repository.;2-8
Abstract: Complex coacervation of alginate-chitosan and alginate-gelatin were used to develop two-coat systems for the encapsulation of Spathoglottis plicata seeds and protocorms (top-shaped structures formed after seed germination of orchids). Both the seeds and the protocorms could withstand the encapsulation treatments with high viability. About 54% of seeds and 40% of large protocorms (1.6-2.0 mm) were able to tolerate a 6-h desiccation treatment. However, viability of the small protocorms (0.7-0.9 mm) was greatly reduced if they were desiccated before encapsulation. Encapsulation after desiccation significantly increased the percentage viability of seeds and protocorms. Treatment with abscisic acid (ABA, 10-5 M) before desiccation and encapsulation resulted in high percentage viability in seeds and large protocorms whereas the small protocorms were found to be less tolerant to the treatments.
Source Title: Biotechnology and Bioengineering
ISSN: 00063592
DOI: 10.1002/(SICI)1097-0290(19980905)59:53.0.CO;2-8
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