Please use this identifier to cite or link to this item: https://doi.org/10.1021/jf8040484
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dc.titleQuantification of antioxidant capacity in a microemulsion system: Synergistic effects of chlorogenic acid with r-tocopherol
dc.contributor.authorSim, W.L.S.
dc.contributor.authorHan, M.Y.
dc.contributor.authorHuang, D.
dc.date.accessioned2014-10-16T08:38:21Z
dc.date.available2014-10-16T08:38:21Z
dc.date.issued2009-05-13
dc.identifier.citationSim, W.L.S., Han, M.Y., Huang, D. (2009-05-13). Quantification of antioxidant capacity in a microemulsion system: Synergistic effects of chlorogenic acid with r-tocopherol. Journal of Agricultural and Food Chemistry 57 (9) : 3409-3414. ScholarBank@NUS Repository. https://doi.org/10.1021/jf8040484
dc.identifier.issn00218561
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/94637
dc.description.abstractWe report herein a characterization of an oil-in-water (o/w) microemulsion consisting of 12% methyl linoleate (or styrene in weight %), 29% surfactant Tween-20, 15% n-butanol, and 44% 75 mM potassium phosphate buffer (pH 7.04). The oil phase droplet size, determined by dynamic light scattering, is 19.3 nm with polydispersity at 0.103. When methyl linoleate is replaced with styrene, the droplet size increases to 63 nm but with much narrower polydispersity at 0.047. The droplet size of styrene is confirmed by polymerization of the styrene in the microemulsion. The polystyrene particles isolated have diameters of ̃70 nm determined by a scanning electronic microscope. Both microemulsions remain stable for two months as expected for a thermodynamically stable system. The methyl linoleate oxidation is induced by AAPH, and the effects of radical scavengers are evaluated in a high throughput fashion using an oxygen sensor coated 96-well microplate Oxygen Biosensor Systems. From the oxygen consumption kinetic curves, the antioxidant capacity can be calculated using Trolox as the standard. The synergistic effect of hydrophilic antioxidants and α-tocopherol was measured for representative flavonoids. Chlorogenic acid has the best synergistic effect of 44.8%. The assay, coined as ORACE standing for oxygen radical absorbance capacity in microemulsion, provides a model system in evaluating antioxidant capacity of phenolic compounds in a heterogeneous system relevant to food and cosmetic applications. © 2009 American Chemical Society.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/jf8040484
dc.sourceScopus
dc.subjectAntioxidant
dc.subjectChlorogenic acid
dc.subjectFree radical
dc.subjectMicroemulsion
dc.subjectSynergistic effect
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.description.doi10.1021/jf8040484
dc.description.sourcetitleJournal of Agricultural and Food Chemistry
dc.description.volume57
dc.description.issue9
dc.description.page3409-3414
dc.description.codenJAFCA
dc.identifier.isiut000265896600003
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